PDF下载 ( 172 KB)
Detection and significance of Helicobacter pyloyi CagA immunoglobulin G in bile of gallstone patients.
-
摘要: 目的探讨幽门螺杆菌（Hp）与胆结石形成的相关性,寻找Hp在胆汁中存在的证据,同时对胆汁中细菌检测方法进行研究分析。方法选择36例胆结石患者,其中28例采用胆管穿刺、8例采用内镜下选择性胆管插管抽取胆汁,用酶联免疫吸附法（ELISA）定量检测胆汁中Hp-CagA IgG,与对照组16例进行比较。结果结石组与对照组、伴有和不伴有急性胆管炎的胆结石患者胆汁中Hp-CagA IgG检出的阳性率均有显著性差异（P<0.05）,而术中胆管穿刺与内镜下选择性胆管插管抽取的胆汁中Hp-CagA IgG检出的阳性率无显著性差异（P>0.05）。结论Hp可能存在于胆道,并与胆结石的形成有一定的关系;经内镜选择性胆管插管抽取胆汁进行Hp-CagA IgG的检测是可行的。
-
关键词:
- 胆结石 /
- 幽门螺杆菌 /
- Hp-CagAIgG
Abstract: Objective To study the relationship between the Helicobacter pylori infection and the formation of gallstones.To explore the evidence of Hp existing and the detective methods of bacteria in biliary tract.Methods Twenty-eight cases of patients with gallstone collected bile by direct puncture during operation and other eight cases with gallstone by ERCP operation.Hp CagA immunoglobulin G detected by enzyme-linked immunosorbent assays (ELISA) .Sixteen cases of patients without gallstone was the control group.Results The detection of Hp-CagA IgG of the two groups has significant difference.There was also significant difference with/without acute cholangitis in biliary tract of the patients with gallstone.However, there was no significant difference between the direct puncture group and ERCP group.Conclusion Hp exists possibly in biliary tract and has some relationship with the formation of gallstone.It's feasible to detect Helicobacter pylori CagA IgG through ERCP.-
Key words:
- Gallstone /
- Helicobacter /
- Hp-CagA IgG
-
[1]Maki T.Pathogenesis of calcium bilirubinate gallstone:role of E.colibeta-glucuronidase and coagulation by inorganic ions, polyelec-trolytes and agitation[J].Annals Of Surgery, 1966, 164 (1) ∶90-100. [2]胡伏莲, 周殿元.幽门螺杆菌感染的基础与临床[M].修订版.北京:中国科学技术出版社, 2002∶30. [3]Sung JY, Leung JW, Olson ME, et al.Demonstration of transientbacterobilia by foreign body implantation in feline biliary tract[J].Digestive Diseases Sciences, 1991, 36 (7) ∶943-948. [4]Figura N, Cetta F, Angelico M, et al.Most Helicobacter pylori in-fected patients have specificantibodies and some also have H.pyloriantigens and genomic material in bile[J].Digestive Diseases Sci-ences, 1998, 43 (4) ∶854-862. [5]郭吕, 唐俐, 郭新瑛, 等.胆结石患者血清和胆汁幽门螺杆菌相关蛋白免疫印迹检测[J].临床消化病杂志, 2002, 14 (4) ∶168-170. [6]Offner GD, Gong D, Afdhal NH.Identification of a 130-kilodaltonhuman biliary concanavalin A binding protein as aminopeptidaseN[J].Gastroenterology, 1994, 106 (3) ∶755-762. -
本文二维码
计量
- 文章访问数: 2214
- HTML全文浏览量: 21
- PDF下载量: 803
- 被引次数: 0
下载:
