慢性乙型肝炎患者外周血中淋巴细胞亚群与肝组织炎症活动度的相关性研究

吴引伟; 文剑; 赵伟; 张亮; 宋玉华; 谈国蕾; 赵磊

慢性乙型肝炎患者外周血中淋巴细胞亚群与肝组织炎症活动度的相关性研究

基金项目:

2003年度南京市医学科技发展项(YKK0328)； 2003年度江苏省社会发展指导性项目(BS2003601)；

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出版历程
- 出版日期: 2008-03-20

Study on the relationship in peripheral blood lymphocyte subsets and inflammation activity in liver tissue in patients with chronic hepatitis B infection

Research funding:

摘要

摘要: 目的探讨慢性乙型肝炎患者外周血中淋巴细胞亚群与肝组织炎症活动度的关系。方法对36例慢性乙型肝炎患者行肝脏穿刺术,对炎症程度进行病理分级,同时应用流式细胞技术测定其外周血单个核细胞膜上相关CD分子的表达情况,并与22例健康对照组进行比较分析。结果15例G0G2期患者外周血中CD4+CD45RO+CD45RA-及CD8+CD45RA+CD62L+淋巴细胞与健康对照组相比具有统计学差异（P<0.05）;14例G3期患者外周血中CD3+、CD3+CD8+、CD8+CD28+、CD8+HLA-DR+CD38+及CD3-CD1+9淋巴细胞与健康对照组相比具有统计学差异（P<0.05）;7例G4期患者外周血CD4+CD45RA+CD62L+及CD1+9CD5+淋巴细胞与健康组相比具有统计学差异（P<0.05）;三组间淋巴细胞数均无统计学差异。不同组别的部分淋巴细胞之间具有一定的相关关系。结论慢性乙型肝炎患者外周血中淋巴细胞亚群数量的改变与病情的轻重有一定的相关性;不同活动期的慢性乙型肝炎患者外周血淋巴细胞之间具有一定的相关关系。
- 慢性乙型肝炎 /
- 淋巴细胞亚群 /
- 流式细胞术 /
- 肝组织 /
- 炎症活动度
Abstract: Objective To explore the relationship in peripheral blood lymphocyte subsets and inflammation activity in liver tissue in patients with chronic hepatitis B infection.Methods 36 liver tissues were procured by hepatic puncture leaded under B-ultrasonic from patients with chronic hepatitis B, and classified as various period by international standardization.The expressions of CD3、CD4 and CD38 etc.in peripheral blood lymphocytes from 36 HBV patients and control group (22 healthy people) were analyzed by flow cytometry (FCM) , and analyzed the correlation with inflammation activity in liver tissue.Results The level of CD+4CD45RO+CD45RA-and CD+8CD45RA+CD62L+expressions in peripheral blood lymphocytes from 15 patients with chronic hepatitis B (G0G2) were higher than that of control group (P<0.05) ;The levels of CD+3、CD+3CD+8、CD+8CD+28、CD+8HLA-DR+CD+38 and CD-3CD+19 expressions in peripheral blood lymphocytes from 14 patients with chronic hepatitis B (G3) were higher than that of control group (P<0.05) ;The levels of CD+4CD45RA+CD62L+ expressions in peripheral blood lymphocytes from 7 patients with chronic hepatitis B (G4) were lower and the levels of CD+19CD+5 expressions were higher than that of control group (P<0.05) .There were no statistics differences in the quantities of peripheral blood lymphocyte subsets in G0G2, G3 and G4.There were positive correlations between the levels of part of CD expressions in the groups.Conclusion There were partially correlation between the quantities of peripheral blood lymphocyte subsets and the condition of illness in patients with chronic hepatitis B infection.There were no significant differences in the quantities and partially correlation between the quantities of peripheral blood lymphocyte subsets in different active periods of the patients with chronic hepatitis B infection.
- chronic B hepatitis /
- lymphocyte subsets /
- flow cytometry /
- liver tissue /
- inflammation activity

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参考文献(8)

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