Study of the relationship between BCP mutation in the hepatitis B virus gene and HBVDNA rebound after lamivudine therapy
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摘要: 目的探讨HBV BCP变异与拉米夫定抗病毒治疗后HBVDNA反弹的关系。方法应用PCR-序列分析法,检测拉米夫定治疗(100mg/d)1年以上,达到病毒学应答半年以上,再出现HBV DNA反弹(HBV DNA拷贝数≥1.0×104拷贝/ml)的27例乙型肝炎患者(治疗组),以及19例从未用过抗病毒治疗患者(对照组)的HBV C区基本核心启动子(BCP)的突变位点。结果1.治疗组HBVDNA反弹的27例BCP(A1762T+G1764A)变异检出率44.44%(12/27)高于对照组26.32%(5/19),但无统计学差异,P>0.05。2.4例HBVDNA反弹患者治疗前未检出BCP(A1762T+G1764A)变异,治疗后有2例检出BCP(A1762T+G1764A)变异。结论BCP(T1762/A1764)变异可能与拉米夫定治疗后HBVDNA反弹有关。Abstract: Objective To investigate the relationship of Hepatitis B virus BCP mutation in the hepatitis B virus (HBV) genome and HBVDNA rebound after with lamivudine therapy.Methods With inspected twenty-seven patients (therapy group, HBVDNA>104 copie4/ml) with HBVDNA rebound after respond to virus beyond 6 months with infecting hepatitis B received lamivudine (100mg/day) oral application for at least 12 months and nineteen patients (control group) with infecting hepatitis B without treated with the drug of antivirus, we have examined mutation in the Basal Core Promoter (BCP) of the Hepatitis B virus (HBV) of two group by PCR-gene sequence analysis.Results 1.The rate of BCP (A1762T+G1764A) mutations were 44.44% (12/27) in HBVDNA-negative group beyond control group 6.32% (5/19) , but it has not statistic difference (P>0.05) .2.In the therapy group, BCP (A1762T+G1764A) mutation were not examined in four patients before therapy, but it were examined in two patients after therapy.Conclusion BCP (T1762/A1764) mutation may be associated with HBVDNA rebound after lamivudine therapy.
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Key words:
- hepatitis B virus /
- BCP mutation /
- Lamivudine /
- HBV DNA rebound
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