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Sequencing and analyzing HBV complete genome sequence of chronic hepatitis B patients with positive HBsAg and anti-HBs simultaneously.
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摘要: 目的报告HBsAg与抗-HBs同时阳性慢性乙肝患者基因序列变化特点。方法对三例HBsAg与抗-HBs同时阳性慢性乙肝患者血清中HBV DNA基因全序列进行PCR扩增、测序,并对测序结果及S区氨基酸进行分析。结果测序结果发现三例患者血清中HBV S区氨基酸均发生替换突变,并且,三例患者中HBV DNA发生碱基替换的位点大部分相同,且替换后碱基基本相同。结论HBsAg与抗-HBs同时阳性慢性乙型肝炎患者常发生S区氨基酸序列突变,双阳现象的出现亦可能与S区外其他位点碱基突变有关。Abstract: Objective To clarity the characteristics of gene sequence of chronic hepatitis B patients with positive HBsAg and anti-HBs simultaneously.Methods The whole HBV DNA sequence was amplified and sequenced from the serum of three chronic hepatitis B patients with positive HBsAg and anti-HBs simultaneously, the results of sequencing and amino acid sequence of S region were analyzed.Results The sequencing results of HBV from the serum of our three patients suggested amino acid substitutions in S region;in addition, the majority of substitution sites of HBV DNA nucleotides and the results of substitution were identical..Conclusion Among the patients, there were often amino acid variations within S region, and the appearance of double positive may also associated with nucleotides mutation at other sites outside S region.
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Key words:
- hepatitis B virus /
- surface antigen /
- surface antibody /
- variation
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