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Influences of Protopanaxdiol to vascular endothelial growth factor and basofibroblast growth factor protein expression of liver
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摘要: 目的探讨20（s）-原人参二醇（Ppd）对肝癌血管内皮生长因子（VEGF）及碱性成纤维细胞生长因子（bFGF）表达的影响。方法建立肝癌动物模型,将实验动物分为五组,每组10只,分对照组、环磷酰胺组（CTX）、20（s）-原人参二醇25mg/kg、50mg/kg、100mg/kg给药组,给药二周后处死动物。称肿瘤重量及肿瘤体积,制成组织切片,以备免疫组化应用。结果与对照组相比,给药组VEGF、bFGF表达受到抑制,肿瘤的重量及体积明显减轻,组间有显著性差异（P<0.01）。结论提示Ppd抑制了VEGF、bFGF蛋白的表达,抑制了肿瘤生长。
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关键词:
- 20（s）-原人参二醇 /
- 肝癌 /
- 血管内皮生长因子 /
- 碱性成纤维细胞生长因子
Abstract: Objective To discuss influences of Protopanaxdiol to vascular endothelial growth factor and basofibroblast growth factor protein expression of liver.Methods To construct liver cancer animal models and the expermental animals are divided into five groups.Each group includes ten animals.That is control group, CTX group, Protopanaxdiol 25mg/kg, 50mg/kg, 100mg/kg groups.After two weeks, all the expertimental animals will be killed.We'll determine tumor volume and weight, then deal it into tissue spices for immunohistochemistry.Results In Protoanaxdiol groups, bFGF and VEGF protein expression is lower than that control group, its tumor volume and weight is lower distinguishly.Conclusion It suggests that Ppd inhibites expression of VEGF and bFGF protein, finally inhibites tumor growth.-
Key words:
- Protopanaxdiol /
- liver cancer /
- VEGF /
- bFGF
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[1]李绪文, 林燕飞, 郑莹.1Rp2HPLC法测定西洋参茎叶皂苷酸、碱降解物中20 (s) 2原人参二醇的含量[J].吉林大学学报, 2006;32 (2) ∶353-6. [2]秦俊杰, 杨艳秋, 李颖新, 等.20 (S) -原人参二醇对肝癌血管密度和肿瘤细胞增殖活性的影响[J].临床肝胆病杂志, 2006, 22 (4) ∶260-1. [3]Lishi H, Tatsuta M, Baba M, et al.Inhibiton by ginsenoside Rg3 ofbomb-esin enhanced peritoneal metastasis of intentional adenocarci-noma in-duced by azoxymethane in Wistar rats[J].Chin Exp Me-tastasis, 1997, 15 (6) ∶603. [4]Hanahan D, Folkman J.Patterns and emergingmechanisms of the an-gio-genic switch during tumorigenesis[J].Cell, 1996, 86 (3) ∶353-64. [5]秦俊杰, 李永杰, 付军, 等.20 (S) 2原人参二醇对肝癌生长的抑制作用及对癌细胞凋亡的影响[J].中国老年学杂志, 2006, 26 (9) ∶1157-8. -
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