实时荧光定量PCR分析慢性丙型肝炎患者胸腺近期输出功能

郭海燕; 陈明; 汪莉萍; 葛国洪; 张言超

实时荧光定量PCR分析慢性丙型肝炎患者胸腺近期输出功能

基金项目:

江苏省科委社会发展计划基金(BS2004014)；

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中图分类号: R512.6
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出版历程
- 出版日期: 2007-06-20

Recent thymic output function in patients with chronic hepatitis C by real time fluorescence quantitative PCR analysis

Research funding:

摘要

摘要: 目的观察慢性丙型肝炎（CHC）患者外周血单个核细胞（PBMCs）中T细胞受体重排删除环（TRECs）的含量,从而推测CHC患者的胸腺近期输出功能,并且比较TRECs含量与HCV载量的关系。方法利用实时荧光定量PCR方法检测22例CHC患者及16例正常人PBMCs中TRECs含量,用RT-PCR方法结合荧光探针的检测技术检测CHC组外周血血清中的HCVRNA。结果CHC患者中TRECs含量为（5.52±1.87）copies/103PBMCs,低于正常人TRECs水平（7.19±2.42）copies/103PBMCs,P<0.05;TRECs含量与HCV载量直线相关分析r=-0.427,P<0.05。结论CHC患者TRECs水平低于正常人,显示其胸腺近期输出功能明显降低,且与HCV载量呈负相关性。
- 慢性丙型肝炎 /
- TRECs /
- 胸腺近期输出功能 /
- 实时荧光定量PCR
Abstract: Objective To analyze the content of T-cell receptor excision circles (TRECs) within peripheral blood mononuclear cells of chronic hepatitis C (CHC) patients to value the thymic output function of CHC and investigate the relation of thymic output function and HCV RNA in chronic hepatitis C (CHC) patients.Methods Quantitative detection of TREcs in DNA of peripheral blood mononuclear cells (PBMCs) from 22cases with CHC, 16 normal individuals was performed by real-time fluorescence quantitative PCR, and detection of HCV RNA of peripheral blood serum in CHC by RT-PCR with fluorescent probe technology.Results The mean of TRECs in patients with CHC (5.52±1.87 copies/103PBMCs) was lower than that in normal individuals (7.19±2.42 copies/103 PBMCs, P<0.05) ;The value of TRECs in patients with CHC was inversely correlated with HCV RNA (γ=-0.427, P<0.05) .Conclusion TRECs levels in CHC are lower in the healthy and inversely correlated with HCV RNA.
- chronic hepatitis C (CHC) /
- TCR rearrangement excision circle (TRECs) /
- recent thymic output function /
- real-time fluorescence quantitative PCR

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参考文献(7)

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