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The applied value of Dane granule antigen of hepatitis B viral in detecting chronic hepatitis B infection
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摘要: 目的分析乙肝病毒五项检测方法和乙肝病毒丹氏颗粒抗原检测试剂在检测慢性乙型肝炎感染中的一致性,探讨丹氏颗粒抗原检测试剂在检测慢性乙肝感染的经济性和可靠性。方法ELISA方法检测134例慢性乙肝患者的血清标志物和丹氏颗粒抗原,荧光定量PCR方法检测HBV DNA。比较各组丹氏颗粒抗原水平及HBVDNA定量水平和乙肝五项的相互关系。结果慢性乙肝患者HBsAg、HBeAg、HBcAb阳性组、HBsAg、HBcAb阳性组、HBsAg、HBeAb、HBcAb阳性组和HBsAb、HBeAb、HBcAb阳性组丹氏颗粒抗原阳性率分别为100.0(、100.0(、94.3(和0。结论丹氏颗粒抗原试剂在检测慢性乙肝感染中与乙肝五项指标及HBV DNA定量之间具有一致性。丹氏颗粒抗原是一个能较好的反应乙肝病毒复制的指标,对乙肝的诊断、预后及抗病毒疗效观察有较好的实用价值。
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关键词:
- 乙型肝炎病毒 /
- 乙型肝炎病毒丹氏颗粒抗原 /
- 血清标志物 /
- 乙肝病毒核酸
Abstract: Objective To analyze the consistency in detecting method of hepatitis B virus (HBV) and Diagnostic Kit for hepatitis B virus Core Antigen and Presl Antigen (Dane granule antigen) .To explore the economy and credibility of Dane granule antigen of HBV in detecting chronic hepatitis B infection.Methods HBV markers、Dane granule antigen of HBV and HBVDNA in 134 patients with chronic hepatitis B were detected respectively by ELISA and fluorescence quantitative PCR.To compare the relationship among Dane granule antigen、HBVDNA and HBV markers in different groups.Result The positive rates of Dane granule antigen of HBV were 100.0%、100.0%、94.3% and 0% respectively in the groups of HBsAg (+) /HBeAg (+) /HBcAb (+) 、HBsAg (+) /HBcAb (+) 、HBsAg (+) /HBeAb (+) /HBcAb (+) and HBsAb (+) /HBeAb (+) /HBcAb (+) .Conclusion Dane granule antigen of HBV has consistency in detecting the infection of chronic hepatitis B with serum markers and HBV-DNA quantitation.It can reflect the replication of HBV and have applied value in the diagnosis of hepatitis B、judgement of prognosis and the curative effect of antivirus.-
Key words:
- hepatitis B virus /
- Dane granule antigen of hepatitis B virus /
- serum markers /
- HBV-DNA
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