前S1抗原在诊断HBeAg阴性慢性乙型肝炎病毒复制中的作用

杨小娟; 吴国荣; 陆忠华; 裴豪; 钱金娟

前S1抗原在诊断HBeAg阴性慢性乙型肝炎病毒复制中的作用

1. 无锡市传染病医院检验科
2. 无锡市第一人民医院

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中图分类号: R512.6
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出版历程
- 出版日期: 2007-05-20

Clinical signification of detecting PreS;antigen for identifying viral replication in patients with HB without HbeAg

摘要

摘要: 目的了解前S1抗原(PreS1Ag)与HBeAg阴性慢性乙型肝炎病毒复制的关系及诊断HBeAg阴性慢性乙型肝炎的价值。方法采用酶联免疫吸附试验(ELISA)方法对150例HBeAg阴性慢性乙型肝炎患者血清进行PreS1Ag测定并与血清HBV DNA做对比分析。免疫组化法检测了HBsAg、HBcAg和PreS1Ag在肝组织的表达。结果血清PreS1Ag和HBV DNA检出率为62.7%和66.0%,x2检验,P=0.398,检出率高度符合,无统计学差异。肝组织HBsAg、HBcAg和PreS1Ag的表达阳性率分别为78.0%、58.7%与61.3%。HBsAg表达阳性率最高。PreS1Ag与HBcAg的表达几乎平行,x2检验,P=0.476,无显著性差异。结论PreS1Ag能够反映HBeAg阴性的慢性乙型肝炎患者的是否有病毒复制。
- 肝炎e抗原,乙型 /
- 病毒复制
Abstract: Objective To investigate the role of hepatitis B virus-preS1 (HBV-preS1) in detecting the replication of virus and predicting the prognosis of HBeAg-negative hepatitis B.Methods Using ELISA method.blood sample of 150 HBeAg-negative hepatitis B patients were analyzed about preS1 and HBVDNA comparatively.HBeAg、HBcAg and PreS1Ag in tissue by immunohistochem is try method.Results The coincidence rates of preS1 and HBVDNA were 62.7% and 66% respectively (P=0.398) .The results of preS1 and HBVDNA detection coincided greatly.Intracellular HBeAg、HBeAg and PreS1Ag account for 78%、58.7% and 61.3% respectively.Positive rate of expressing HBeAg, PreS1Ag are little less than HBeAg, but near between PreS1Ag and HBcAg.There are no significant difference by x2 test (P=0.476) .Contlusion PreS1 can reflect the relication of HBV sensitively for those who are HBeAg-negative.
- hepatitis B e antigens /
- virus replication

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参考文献(5)

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[2] 中华医学会.病毒性肝炎防治方案[J].中华肝脏病杂志, 2000, 8 (6) ∶324-329.

[3]Akarca US, Greene S, Lok AS.Detection of precore hepatitis B vi-rus mutants in asmptomatic HBsAg-positive family members[J].Hepatology, 1994, 19∶1366-1370.

[4]李琴, 孙桂珍, 魏玉香, 等.前蛋白与病毒DNA和核心抗原对乙型肝炎病毒复制诊断的对比[J].中华肝脏病杂志, 2004, 12∶134-136.

[5]Funk ML, Rosenberg DM, Lok AS.World-wide epidem iology ofHBeAg-negative chronic hepatitis B and associated precore and corepromoter variants[J].J Viral Hepat, 2002, 9∶52-61.

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