Analysis of differential gene expression profiles in hepatic fibrosis tissue using cDNA microarray
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摘要: 应用DNA微阵矩技术研究乙型肝炎肝硬化组织差异基因表达谱改变,以寻找肝纤维化相关基因,探讨肝纤维化的发病机理。抽提正常肝组织和肝硬化组织中的mRNA来制备探针,经杂交、洗涤后,通过计算机扫描分析正常肝组织和肝硬化组织基因表达谱的差异情况。在10000个候选基因中,筛选出99条差异表达基因,表达上调的有45条,表达下调的有54条。其中未知基因9条。因此基于DNA微阵矩技术的肝纤维化基因表达谱分析能够高通量筛选与肝纤维化发生发展相关的基因。Abstract: To investigate the differentially expressed genes in hepatic fibrosis tissues using cDNA microarray and screen for fibrosis-related genes.Total RNA was extracted from normal human liver tissues and liver cirrhosis tissues.The mRNA was used to prepare probes.The mixed probes were hybridized to the cDNA microarray.After washing, the DNA chips were scanned by laster scanner.The acquired image was analyzed.Among the 10.000 target genes, 99 genes were identified in liver cirrhosis tissues that were either over or under-expressed as compared to controls.45 genes were up-regulated and 54 down-regulated.9 new genes were found in the study.The results showed that the cDNA microarray for analysis of gene expression profile is a powerful method for screening hepatic fibrosis-related genes.
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