The relationship between HLA-A alleles polymorphism and susceptibility to HBV in the Han nationality in Xuzhou
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摘要: 探讨慢性乙型肝炎与HLA-A位点的等位基因多态性。用PCR-SSP方法对徐州地区汉族正常组和慢性乙型肝炎组的HLA-A基因多态性进行分析。HLA-A*0201-17出现的频率在慢性乙型肝炎组为45.57%,高于正常组的29.69%,P=0.002<0.05,RR=1.983,(95%C I:1.227-3.080),具有统计学意义。HLA-A*0201-17与慢性乙型肝炎较高的相关性,可能为易感基因。
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关键词:
- 乙型肝炎 /
- 疾病易感性 /
- 人类白细胞抗原 /
- 序列特异性引物聚合酶链反应
Abstract: To investigate the relationship between HLA-A alleles polymorphism and susceptibility to HBV in the Han nationality in Xuzhou.Typing HLA-A genotype was performed by using PCR-SSP (polymerase chain reaction-sequence specific primers technique) in seventy-nine patients with hepatitis B and ninty-six healthy people in Xuzhou of China.The frequency of HLA-A*0201-0217 allele was 45.57% in patient group, which was higher than that (29.69%) of the normal healthy people (x2=9.394, P<0.05, RR=1.983, 95%CI:1.2273.080) .The susceptibility to chronic hepatitis B might be strongly associated with HLA-A*0201-0217 allele of Chinese patients in Xuzhou.-
Key words:
- hepatitis B /
- susceptibility /
- human leukocyte antigen /
- PCR-SSP
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[1]梁晓峰, 陈园生, 王晓军, 等.中国3岁以上人群乙型肝炎血清流行病学研究[J].中华流行病学杂志, 2005, 26 (9) ∶655-658. [2]Galan MV, Boyce D, Gordon SC.Current pharmacotherapy for hepa-titis B infection[J].Expert Opin Pharmacother, 2001, 2∶1289-1298. [3]Jung MC, Pape GR.Immunology of hepatitis B infection[J].Lancetinfect Dis, 2002, 2∶43-50. [4]Bunce M, O'Neill MO, Barnardo MCNM, et al.Phototyping:com-prehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5&DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers (PCR-SSP) [J].Tissue Antigens, 1995, 46∶355-367. [5]Bodmer J G, Marsh S G E, Albert D A, et al.Nomenclatrue for fac-tors of the HLA-A system[J].Tissue Antigen, 1997, 49∶297. [6]The MHC sequencing consortium.Complete sequence and gene mapof a human major histocompatibility complex[J].Nature, 1999, 401 (6756) ∶921-3. [7]陈兰羽, 陈建杰, 章晓鹰.HLA-A、DRB1等位基因多态性与上海汉族人乙肝后肝硬化相关性研究[J].胃肠病学和肝病学杂志, 2005, 14 (5) ∶487-489. [8]Karan MA, Tascioglu NE, Ozturk AO, et al.The role of HLA anti-gens in chronic hepatitis B virus infection[J].J Pak Med Assoc, 2002, 52∶253. [9]明利华, 朱源荣Gurtis CH, 等.HLA-A02基因型与中国高发地区肝细胞癌密切关联[J].中国免疫学杂志, 1999, 15∶305-307.
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