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The correlation of α-SMA and FAK with hepatic fibrosis after chronic hepatitis B
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摘要: 探讨α-平滑肌肌动蛋白（α-SMA）和粘着斑激酶（FAK）与慢性乙型肝炎病理肝纤维化分期（S）之间的相关性。选取行肝组织活检的慢性乙型肝炎患者42例,对肝组织进行HE、Masson三色、网状纤维染色,并进行肝纤维化分期（S0-S4）。免疫组化法测定肝组织中α-SMA和FAK,并进行半定量分析。不同分期的肝纤维化α-SMA、FAK表达积分情况不同（P<0.01）,与S0（0.429±0.535,0.143±0.378）比较,S1（1.571±1.397,1.286±1.113）、S2（2.875±1.885,1.875±1.885）、S3（4.286±0.756,4.286±0.756）、S4（4.796±0.927,4.923±0.954）积分明显逐渐升高（P<0.05）,具有S4>S3>S2>S1>S0的规律。相关性分析显示α-SMA、FAK表达积分与肝纤维化分期（S）呈正相关（P<0.05）。肝组织中α-SMA、FAK的表达在肝纤维化发生发展过程中发挥重要作用。Abstract: To explore the correlation of α-SMA, FAK and the hepatic fibrosis after hepatitis B.Liver biopsies were obtained from 42 cases with hepatitis B.The liver tissues were treated with HE, Masson and V-G, and were classified for stages of liver fibrosis.The expression of α-SMA and FAK in fibrotic liver tissue was observed by immune histochemical techniques, and analyzed in half-ration.The level of α-SMA and FAK were different in various stages of hepatic fibrosis (P<0.01) .After analyzing S0 (0.492±0±535 and 0.143±0.378) , S1 (1.571±1.397 and 1.286±1.113) , S2 (2.875±1.885 and 1.875±1.885) , S3 (4.286±0.756 and 4.286±0.756) , and S4 (4.796±0.927 and 4.923±0.954) , it showed that the scores were increased significantly (P<0.05) .α-SMA and FAK were positive related to the stages of hepatic fibrosis (P<0.05) .The expression of α-SMA and FAK might take important action on the process of the hepatic fibrosis.
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Key words:
- α-smooth muscle actin /
- focal adhesion kinase /
- hepatic stellate cells /
- hepatic fibrosis
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