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人胎肝干细胞体外分离、培养及鉴定

高沿航 李玉林 李艳茹 吴珊 全成实 王芯蕊 张丽红

引用本文:
Citation:

人胎肝干细胞体外分离、培养及鉴定

基金项目: 

“863”计划重大专项(2004AA205020); 

详细信息
  • 中图分类号: R329

Isolation, culture, identification of human fetal liver stem cells in vitro

Research funding: 

 

  • 摘要: 研究人胎肝干细胞体外分离纯化方法,并对其进行初步鉴定及生物学特性分析。采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞,通过免疫细胞化学方法对其进行初步鉴定,以及应用流式细胞仪等对其生长状况进行评估。所获得的原代细胞共传8代,细胞呈小梭形或三角形,体积较小,胞核较大,而胞浆较少;第4代细胞中进入生长期的细胞约占88.2%;免疫细胞化学染色显示细胞胞质中ALB、CK19染色阳性。人胎肝中存在具有肝干细胞特征的细胞,它们可能会成为细胞移植及肝移植良好的细胞资源。

     

  • [1]Bilir BM, Guinette D, Karrer F, et al.Hepatocyte transplantation inacute liver failure[J].Liver Transpl, 2000, 6∶32-40.
    [2]Strom SC, Chowdhury JR, Fox IJ.Hepatocyte transplantation for thetreatment of human disease[J].Semin Liver Dis, 1999, 19∶39-48.
    [3]Kobayashi N, Ito M, Nakamura J, et al.Hepatocyte transplantationimproves liver function and prolongs survival in rats with decompen-sated liver cirrhosis[J].Transplant Proc, 1999, 31∶428-429.
    [4]Kobayashi N, Fujiwara T, Westerman KA, et al.Prevention of acuteliver failure in rats with reversibly immortalized human hepatocytes[J].Science, 2000, 287∶1258-1262.
    [5]Weglarz TC, Degen JL, Sandgren EP.Hepatocyte transplantation in-to diseased mouse liver.Kinetics of parenchymal repopulation and i-dentification of the proliferation capacity of tetraploid and octaploidhepatocytes[J].Am J Pathol, 2000, 157∶1963-1974.
    [6]Selglen PO.Preparation of isolated rat liver cells[J].Methods CellBiol, 1976, 13∶29-83.
    [7]Suzudi A, Zheng Y, Kondo R, et al.Flow-cytometric separationand enrichment of hepatic progenitor cells in developing mouse liver[J].HEPATOLOGY, 2000, 32∶1230-1239.
    [8]Kubota H, Reid LM.Clonogenic hepatoblasts, common precursorsfor hepatocytic and biliary lineages, are lacking classical major histo-compatibility complex classⅠantigen[J].Proc Natl Acad Sci USA, 2000, 97∶12132-12137.
    [9]Hirose T, Yasuchika K, Fujikawa T, et al.“Blastophere”:a newculture method for human fetal hepatic progenitor cells[J].Gastroen-terology, 2002, 120 (Suppl1) ∶A-542.
    [10]Kamiya A, Kojima N, Kinoshita T, et al.Miyajima maturation offetal hepatocytes in vitro by extracellular matrices and oncostatin M:induction of tryptophan oxygenase[J].HEPATOLOLY, 2002, 35∶1351-1359.
    [11]张淑莲, 曾平鲁, 肖荣, 等.肝干细胞分离培养条件的筛选[J].肝脏, 2003, 8 (1) ∶31-32.
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  • 出版日期:  2007-01-20
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