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The effect of TNF-α on express of MMPs and IL-8 of cultured hepatic stellate cell in human liver.
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摘要: 了解TNF-α对人肝星状细胞表达MMPs和IL-8的影响。用TNF-α刺激培养的hHSC,用ELISA检测不同时间培养上清中的MMPs和IL-8的浓度。结果显示受TNF-α刺激后,MMP-1、MMP-2和IL-8水平为28h>24h>20h>4h—16h（P<0.050.001）;MMP-3为28h>24h（P<0.01）,24h>20h>4h—16h（P<0.05）。TNF-α刺激后MMP-1和MMP-2产生较早,从8h起已高于对照组（P<0.05）,而MMP-3的平均浓度,从20h起才高于对照组（P<0.01）。提示TNF-α可刺激hHSC表达MMP-1、MMP-2、MMP-3和IL-8,其表达水平随刺激时间的延长而逐渐增加;未受TNF-α刺激的hHSC可表达一定量的MMPs,基本不表达IL-8。Abstract: To probe the effect of TNF-α on express of matrix metalloproteinases (MMPs) and IL-8 in a culture medium of human hepatic stellate cells (hHSC) .hHSC were cultured and stimulated by TNF-α.MMPs and IL-8 in a culture were detected by ELISA at different time after TNF-α stimulation.The results shown that MMP-1, MMP-2 and IL-8 level was in turn at 28h>24h>20h>4h—16h (P<0.05-0.001) , MMP-3 at 28h>24h (P<0.01) >20h>4h—16h (P<0.05) .Average level of MMP-1 and MMP-2 in TNF-α group was higher than control after a 8 hours stimulation respectively (P<0.05) , and average level of MMP-3 was not significantly higher than control till at 20h (P<0.01) .The present data indicated that TNF-α could stimulate hHSC to express MMP-1, MMP-2, MMP-3 and IL-8, and hHSC not stimulated by TNF-α may express a little MMPs but no IL-8.
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Key words:
- hepatic stellate cell /
- TNF-α /
- IL-8 /
- matrix metalloproteinases
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