Abstract: To study the relation between the change of concentration of complement 3 (C3) 、transferrin (Tf) and α1-antitrypsin (α1-AT) in sera of patients with hepatitis B and the damage of liver.healthy adults are used as controls.Patients with hepatitis B are divided into four groups: Actue hepatitis (aH) 、chronic hepatitis (CH) 、liver cirrhosis (LC) and liver cancer (HCC) .Detect the concentration by ratenephelometer.Results C3: All groups of hepatitis B are lower than the control group.AH、CH and HCC are higher than LC (P<0.01) , The differences are significant.Tf: All the groups of hepatitis B are lower than the control group, the differences are significant (P<0.01) .AH and CH are higher than LC or HCC, The differences are significant. (P<0.01) α1-AT: AH and HCC are higher than control (P<0.01) ;HCC is higher than CH. (P<0.05) ;The differences are significant.There is a significance to observe the degree of the damage of liver and the course of hepatitis detecting the concentration of C3、Tf and α1-AT in sera of patients with hepatitis B.