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The relation of chronic hepatitis C and relapse with HCV RNA in sera and peripheral blood mononuclear cells
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摘要: 通过对外周血单个核细胞（PBMC）中正链及负链HCV RNA的检测来探讨HCV在PBMC中的复制与丙肝慢性化及复发的关系。应用巢式RT-PCR对71例慢性丙型肝炎患者的血清和PBMC进行正负链HCV RNA的检测,其中15例干扰素治疗。并对10例正常人的血清和PBMC进行了HCV RNA检测。71例慢性丙型肝炎患者血清中正链HCV RNA阳性率73.2%,负链HCV RNA阳性率为0,PBMC中正链HCV RNA阳性率为60.5%,负链HCVRNA阳性率为38.0%。15例干扰素治疗患者在治疗后血清正链HCV RNA转阴率为73.3%,PBMC中正链HCVRNA转阴率为45.5%,负链转阴率为71.4%。正常对照组血清及PBMC中HCV RNA阳性率为0。这说明HCV RNA在PBMC中的复制与丙肝慢性化及复发存在一定的关系。干扰素治疗丙肝具有一定疗效,但不能彻底清除病毒。Abstract: To study the relation of chronic hepatitis C and relapse with HCV RNA in sera and peripheral blood mononuclear cells (PBMC) .Sera and PBMC of 71 patients (cases treated with IFN) with chronic hepatitis C and 10 healthy people were separated. HCV RNA was detected with reverse transcription and polymerase chain reaction.Positive strants of HCV RNA were detected in 73.2 percent of sera and all negative strants in sera were negative.Positive rates of positive and negative strants of HCV RNA in PBMC were 60.5 percent and 38.0 percent.When the IFN therapy was finished, 73.3 percent positive strants in serabecame negative.45.5 percent positive strants and 71.4 percent negative strants in PBMC became negative.Positive and negative strants of HCV RNA were not detected in sera and PBMC of healthy people.It might be a cause of chronic hepatitis C that HCV can infect PBMC and replicate in these cells.IFN have some cure to hepatitis C but cant clear HCV RNA.
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