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Analysing the change of HBV DNA in children with hepatitis B
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摘要: 了解小儿乙型肝炎病毒(HBV DNA)复制水平的变化及其临床意义。采用微粒子酶免分析法(MEIA)检测HBVM,荧光定量PCR法检HBV DNA。乙肝患儿血清HBeAg滴度与HBV DNA密切相关(P<0.005)。HBsAg,ALT与HBV DNA则无明显相关性。HBV DNA定量检测,能准确地评价HBV在宿主体内的复制情况,可作为临床上评价病毒复制程度和抗病毒疗效的指标。Abstract: To investigate the change of HBV DNA in children with hepatitis B.HBVM and HBV DNA were detected by microparticle enzyme immunoassay (MEIA) and PCR respectively.The level of HBeAg and HBV DNA were correlated strongly (P<0.005) , but there were no relationshinp between HBsAg and HBV DNA, ALT and HBV DNA (P>0.05) .The level of HBV DNA was the lowest in severe degree patients among that chronic hepatitis B.Quantitative detection of HBVM and HBV DNA can evaluate exactly the reproduction of HBV in the children with hepatitis B.They are most powerful predictor in defining disease progression and following response to therapy in HBV infection.
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Key words:
- children /
- hepatitis B /
- HBVDNA
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[1] 中华医学会.病毒性肝炎防治方案[J].中华肝脏病杂志, 2000, 8 (6) ∶324-329. [2]Cho SC, Lee SH, Shinn JJ, et al.HBV DNA levels, aminotransferaseand histological activity in young male patients with HBeAg positivechronic Hepatitis B[J].Taehan Kan Hakhone Chi, 2002, 8 (1) ∶44-51. [3]陈成伟.乙型肝炎病毒血清标志物的临床意义及评价[J].中国实用内科杂志, 2002, 22 (1) ∶52-53. [4]Ohkubo K, Kato Y, Ichikawa T, et al.Viral load is a significant prognos-tic factor for hepatitis B virus-associated hepatocellular carcionma[J].Cancer, 2002, 94 (10) ∶2663-2668. [5]Gowans EJ.Relationship between HBeAg and HBV DNA in patients withacute and persistent hepatitis B infection[J].Med J Aust, 1986, 145 (9) ∶439-441. [6]Guillou D B, Duclos-vallee J C, Eberle F, et al.Evaluation of enzyme-linked immunosorbent assay for detection and quantification of hepatitisB virus preS1 envelope antigen in serum samples∶comparison with twocommercial assays for monitoring hepatitis B virus DNA[J].J Viral Hep-at, 200, 7 (5) ∶387-392. -
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