α-SMA在慢性乙型病毒性肝炎肝组织中的表达

王欣欣; 郎振为; 孟忻; 孙琳; 王泰龄; 赵景民; 张世杰

α-SMA在慢性乙型病毒性肝炎肝组织中的表达

基金项目:

北京市科技计划重大项目资助项目 (H0 2 0 92 0 0 2 0 2 90)；

详细信息

中图分类号: R512.62
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出版历程
- 出版日期: 2005-02-20

Detection of α-smooth-muscle actin in liver tissue of chronic hepatitis B.

Research funding:

摘要

摘要: 探讨慢性乙型病毒性肝炎（CHB）患者肝组织中,α-SMA的表达对肝脏炎性损伤和肝纤维化程度的病理诊断意义。采用免疫组化技术,观察12 7例CHB患者肝穿刺组织中α-SMA的表达,结果与现行病理诊断标准慢性肝炎分级、分期及Knodell-HAI评分系统进行统计学比较。另有6例作为对照,其中急性肝炎3例,静止性肝硬化3例。α-SMA在CHB、急性肝炎及静止性硬化三组中均有表达。主要定位在扩大的汇管区及其周围,以及小叶内点灶状坏死部位。且α-SMA表达与慢性肝炎病理分级（G）、分期（S）及Knodell-HAI评分呈显著正相关。α-SMA是判断慢性乙型肝炎肝损伤程度及纤维化程度的一个重要指标。
- 慢性乙型病毒性肝炎 /
- α-平滑肌肌动蛋白 /
- 免疫组化
Abstract: To study the expressio n of α-smooth-muscle actin and its pathological significance in evaluation of liver fibrosis and neeroinflammation of CHB.Liver biopsy specimens of 127cases of SHB were immunohistochemically stained for α-smooth-muscle actin (α-SMA) to compare the relationship of its expression with histological grading and staging of chro nic hepatitis and the histological activity index of Knodell.Another 6 cases, inc luding 3 cases of actue hepatitis and 3 cases of non-active cirrhosis respective ly, were chosen as controls.α-SMA was detected in all cases, either in CHB or in acute hepatitis and non-active cirrhosis.The cells positive for α-SMA increased around the enlarged portal areas mainly, as well as in some cases of lobular nea r focal necrosis and associated with progression of histological grading, staging and histological activity index of Knodell.α-SMA is an useful marker to judgem ent the degree of liver pathological changes not only for fibrosis but also for necroinflammation of CHB.
- CHB /
- liver fibrosis /
- α-smoo th muscle actin

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参考文献(12)

[1] LevyMT , McCaughanGW , MarinosG , etal.IntrahepaticexpressionofthehepaticstellatecellmarkerfibroblastactivationproteincorrelateswiththedegreeoffibrosisinhepatitisCvirusinfection[J].Liver, 2002, 22∶93-101.

[2] TakuyaKitada, ShuichiSeki, KazuoIKeda, etal.Clinicopathologicalchar acterizationofprion∶anovelmarkerofactivatedhumanhepaticstellatecells[J].JHepatolo, 2000, 33∶751-757.

[3] Schmitt-GraffA , KrugerS , BochardG , etal.Modulationofalphasmoothmuscleactinanddesminexpressioninperisinusoidalcellsofnormalanddiseasedhumanlivers[J].AmJPathol, 1991, 138∶1233-1242.

[4]YamaokaK , NouchiT , MarumoF .Alpha-smooth-muscleactinexpressinnormalandfibrotichumanlivers[J].DigSci, 1993, 38 (8) ∶1473-1479.

[5] ZeibergM , StrutzF , MullerGA , etal.Roleoffibroblastactivationinin ducinginterstitialfibrosis[J].JNephrol, 2000, 13 (suppl3) ∶S111-120.

[6] KhanMA , PoulosJE , BruntEM , etal.Hepaticalpha-smoothmuscleactinexpressioninhepatitisCpatientsbeforeandafterinterferontherapy[J].Hepatogastroenterology, 2001, 48 (37) ∶212-215.

[7] CloustonADJonssonJR , PurdieDM , etal.Steatosisandchronichepati tisC∶analysisoffibrosisandstellatecellactivation[J].JHepatol, 2001, 34 (2) ∶314-320.

[8] UtsunomiyaY , KawamuraT , AbeA , etal.Significanceofmesangialex pressionofalphasmoothmuscleactinintheprogressionofIgAnephropa thy[J].AMJkidneyDis, 1999, 34∶902-910.

[9] PhanSH .Roleofthemyofibroblsatinpulmonaryfibrosis[J].KidneyInt, 1996, 49 (suppl54) ∶S46-48.

[10]陈文笔, 严家春, 田瑞霞, 等慢性乙型肝炎肝组织中TGF -β1及α-SMA的表达变化[J].解放军医学杂志, 2003, 28 (3) ∶273-274

[11]梁英锐, 黄政治乙肝肝硬化碎屑状坏死病变的ISEL及CK -7、α-SMA免疫组化研究[J].胃肠病学和肝病学杂志, 2002, 11 (1) ∶45-49

[12] SvegliatiBaroniG , PatorelliA , ManzinA , etal.Hepaticstellatecellac tivationandliverfibrosisareassociatedwithnecroinflammatoryinjuryandTh1-likeresponseinchronichepatitisC[J].Liver, 1999, 19∶212-219.

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