Abstract: To explore the infection of HBV among pregnant women in the minority nationality regions.Tested HBsAg, HBsAb, HBeAg, HBeAb and HBcAb of 1571 pregnant women by ELIS A, who were in the maternity of three hospitals in the minority nationality regio ns.The infective ratio of HBV of pregnant women in the minority nationality regi ons was 21.26%, Han nation was higher than Tujia and Miao, countryside was higher than city, gravidas whose knowledge below the junior middle school level is high er than whose knowledge above the senior middle school level, multi-pregnancy was higher than one-pregnancy and two-pregnancy.The ratio of HBV infection of pregn ant women in the minority nationality regions is different among Tujia, Miao and Han nation, and it is related to gravidas origin, level of culture and times of pregnancy.