利用组织芯片技术分析肝细胞癌组织P21WAF1/CIP1、PCNA和Ki-67的表达及意义

朱争艳; 高英堂; 杜智; 徐东波; 齐之丽; 景丽; 张文

利用组织芯片技术分析肝细胞癌组织P21WAF1/CIP1、PCNA和Ki-67的表达及意义

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中图分类号: R735.7
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出版历程
- 出版日期: 2003-04-20

Expression of P21WAF1/CIP1, PCNA, Ki-67 in HCC by tissue chip assay

摘要

摘要: 利用组织芯片技术检测P2 1WAF1/CIP1、PCNA与Ki - 6 7在肝细胞癌组织中的表达与分布 ,初步探讨P2 11WAF1/CIP1、PCNA与Ki- 6 7在肝细胞癌发生机制以及预后评估方面的作用。采用免疫组化技术结合临床病理资料检测了 6 1例肝癌和 5 0例癌旁组织的P2 1WAF1/CIP1、PCNA、Ki- 6 7的表达情况。PCNA和Ki- 6 7表达与肝癌的分化程度相关 ,PCNA与肝癌的侵袭转移潜力有关（P <0 0 5 ） ,P2 1WAF1/CIP1在实验中未见有明显差异 ,但在表达程度与肝癌组织的分化程度之间亦存在着一定的相关关系。应用组织芯片高效检测临床组织样本具有快速、方便、经济、准确的优点。P2 1WAF1/CIP1、PCNA与Ki- 6 7的异常表达可能与肝癌的发生发展密切相关
- 组织芯片 /
- P21WAF1/CIP1 /
- PCNA /
- Ki-67 /
- 肝细胞癌
Abstract: To detect the expressions and significance of P21 WAF1/CIP1, PCNA, Ki-67 in HCC by tissue microarray assay. Immunohistochemic stain combined with histological pathology were used to observe the P21 WAF1/CIP1, PCNA and Ki-67 expressions in 61 cases of HCC and 50 cases of peritumorous tissues. The expressions of PCNA, Ki-67 were correlated with histological differentiation, whereas, the expressions of PCNA had relation with potential of invasion and metastasis of HCC. Expressions of P21 WAF1/CIP1, PCNA and Ki-67 may be related to the development of hepatocellular carcinoma and it is feasible to utilize tissue microarray for rapid, economic and accurate screening of clinical tissue specimens on a large scale.
- tissue microarray /
- P21 WAF1/CIP1 /
- PCNA Ki-67 /
- hepatocellular carcinoma /

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参考文献(6)

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