弹力蛋白酶mRNA在肝细胞中的表达

蒋健

弹力蛋白酶mRNA在肝细胞中的表达

蒋健

上海中医药大学附属曙光医院内科上海200021

详细信息

中图分类号: R346
计量
- 文章访问数: 2150
- HTML全文浏览量: 18
- PDF下载量: 824
- 被引次数: 0
出版历程
- 出版日期: 2002-06-20

Elastase mRNA expression of hepatic cells

Jiang Jian

摘要

摘要: 探讨肝脏组织细胞弹力蛋白酶mRNA的表达。克隆成地高辛素标记的大鼠胰腺丝氨酸弹力蛋白酶RNA探针 ,并以此进行原位杂交。多数肝细胞的胞浆内有弹力蛋白酶mRNA的表达 ,其强度因小叶内肝细胞部位的不同而有所差异。组成肝窦窦壁的内皮细胞以及其他细胞也有弹力蛋白酶mRNA的表达。从形态学证实肝脏细胞有弹力蛋白酶mRNA的表达 ,丝氨酸弹力蛋白酶有可能参与肝纤维化细胞外基质代谢
- 弹力蛋白酶 /
- 原位杂交 /
- 肝细胞
Abstract: To investigate elastase mRNA expression of hepatocyte. The probe of rat pancreatic elastase Ⅱ marked by digoxigenin was cloned. The expression of hepatic elastase mRNA was observed by in situ hybridization. The expression of elastase mRNA was found in most hepatic cytoplasm. The level of its expression varies in different hepatocytes situated in the same liver lobule. In endotheliocyte and other cells of hepatic sinusoid, the expression of elastase mRNA was also discovered. It is a new discovery to prove the expression of hepatocyte elastase mRNA from morphology. It can be infered that it is related to metabolism of hepatic ground substance. It may be of value to study the development and degradation of hepatic fiber.
- Elastase /
- In situ hybridization /
- Hepatocyte

HTML全文

参考文献(11)

[1]MacDonald RJ, Swift GH, Quinto C, et al. Primary structure of two distinct rat pancreatic preproelastase determined by sequence analysis of the complete cloned messenger ribonuleic acid and sequences[J].Biochemistry, 1982, 21∶1453-1463.

[2]Schwartz DE, Paller AS, Lizak PP, et al. Elastase and neutral cathepsin production by human fibroblasts:effect of culture condition on synthesis and secretion[J]. J. Invest. Dermatol, 1986, 86∶63-68.

[3] Janoff A, Scherer J. Mediators of inflammation in leukocyte lysosomes Ⅸ. Elastinolytic activity in granules of human polymorphonuclear leukocytes[J].J Exp Med, 1968, 128∶1137-1151.

[4]Werb Z, Gordon S. Elastase secretion by stimulated macrophages:Characterization and regulation[J]. J Exp Med, 1975, 142∶361-377.

[5]Lavie G, Zucker-Franklin D, Franklin EC. Elastase-type proteases on the surface of human blood monocytes:possible role in amyloid formation[J].J Immunol, 1980, 125∶175-180.

[6] Jiang J, Mistumata M, Shu Q, et al. Migration and proliferation of cell for arterial intimal growth[C].Molecelular Biology Intelligence Unit 5. Molecelular Mechanisms of Smooth Muscle Contraction, 1999, ppl 143-161.

[7]Mainardi CL, Dixit SN, Kang AH. Degradation of type Ⅳ (basement membrane) collagen by a proteinase isolated from human polymorphonuclear leukocyte granules[J].J Biol Chem, 1980, 255∶5435-5441.

[8]MacDonald JA, Kelly DG. Degradation of fibronection by human leukocytes elastase[J]. J biol chem, 1980, 255∶8848-8858.

[9]Heck LW, Blackburn WD, Irwin MH, et al. Degradation of basement membrane laminin by human neutrophil elastase and cathepsin G[J].AM J Pathol, 1990, 136∶1267-1274.

[10]Roughleg PJ, Barrett AJ. The degradation of cartilage proteoglycans by tissue proteinases[J].Biochem J, 1997, 167∶629-637.

[11]Murakami T, Sato M, Higashikawa F, et al. Hepatic clearance of ONO-5046, a novel neutrophil elastase inhibitor, in rats and in the rat perfused liver[J]. J Pharm Pharmacol, 1998, 50 (4) ∶425-430.

施引文献

资源附件(0)

访问统计