核酸杂交检测HBV DNA及基因分型

石之嶙; 李体远; 戴勇; 黄瑞芳; 杜珙; 傅蓉

核酸杂交检测HBV DNA及基因分型

详细信息

中图分类号: R346
计量
- 文章访问数: 2303
- HTML全文浏览量: 10
- PDF下载量: 985
- 被引次数: 0
出版历程
- 出版日期: 2002-06-20

Detection and genotyping of HBV DNA by polymerase chain reaction and hybridization

摘要

摘要: 检测血清中HBVDNA并进行基因分型。采用聚合酶链反应 (PCR)扩增HBVDNA后 ,采用不同的探针 ,利用微板杂交法将其分为六个亚型。 5 0例广东地区HBVDNA阳性病人中C型占 6 8% (34例 ) ,B型占 10 % (5例 ) ,D型占 6 % (3例 ) ,F型占 2 % (1例 ) ,另有 8%为混合型 (3例BC ,1例BCD) ,没有发现A型和E型 ,3例分型结果阴性 ,为未定型。广东地区HBV基因亚型以C型为主 ,B型次之 ,其它较少。可能存在其他亚型
- 乙型肝炎病毒 /
- 基因型 /
- 聚合酶链反应 /
- 核酸杂交
Abstract: To detect HBV DNA and its genotypes. HBV DNA and its genotype was detected by Polymerase Chain Reaction and Nucleic Acid hybridization. Of 50 HBV DNA positive patients who lived in Guangdong, 34 samples (68%) are type C, 5 samples (10%) type B, 3 samples (6%) type D, 1 sample (2%) type F, 4 samples (8%) mixed type (3 BC, 1 BCD) , meanwhile 3 samples (6%) couldn't be typed by this method. In the detected samples, the major genotype of HBV DNA is type C, several are type B, D or F. The type A and E haven't been found. The result suggests there may be some other genotypes in Guangdong area.
- hepatitis B virus /
- genotype /
- PCR /
- nucleic acid hybridization

HTML全文

参考文献(5)

[1] OnoY .Ondo, SasadaRetal.ThecompletenucleotidesequencesoftheclonedhepatitisBvirusDNAsubtypeadrandadw[J].NucleicAcidsRes, 1983, 11∶1747～1749.

[2]LindhM , AnderssonAs, GusdalA .Genotypes, nt1858variants, andgeographicoriginofhepatitisBvirus-large-scaleanalysisusinganewgenotypingmethod[J].JInfectDis, 1997, 175∶1285～1293.

[3]朱冰, 骆抗先, 侯金林等检测HBVDNA基因型的方法学探讨[J].第一军医大学学报, 1998, 18∶196～198.

[4]LindhM , GonzalesJE , NorkrandG , etal.GenotypingofhepatitisBvirusbyrestrictionpatternanalysisofapre-samplicon[J].JVirolMethods, 1998, 72 (2) ∶163～174.

[5]ThurszMR , KwiatkowskiD , Allsopp, etal.AssociationbetweenandMNCclassⅡalleleandclearanceofhepatitisBvirusintheGambia[J].NEnglJMed, 1995, 232∶1065～1069.

施引文献

资源附件(0)

访问统计