10-23DNA酶体外切割HBV前C/C区mRNA的初步研究

郑锦辉; 王峰; 牛俊奇

10-23DNA酶体外切割HBV前C/C区mRNA的初步研究

详细信息

中图分类号: R346
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出版历程
- 出版日期: 2002-06-20

The cleavage of PreC/C mRNA of HBV in vitro by a 10-23 DNA zyme

摘要

摘要: 探讨 10 - 2 3DNA酶对HBV前C/C区mRNA的体外切割活性。用克隆及亚克隆技术将HepG2 2 15细胞中HBV前C/C区基因克隆入pCDNA3 1（+）载体 ,转录表达该区mRNA。合成针对HBV前C/C区 2 0 31位点的 10 -2 3DNA酶 ,观察其对靶mRNA的切割活性。 10 - 2 3DNA酶对HBV前C/C区mRNA的切割效率与Mg2 + 离子浓度呈正相关。 10 - 2 3DNA酶能有效切割HBV前C/C区mRNA。
- DNA酶 /
- 乙型肝炎病毒
Abstract: To explore the use of synthetic 10-23DNA zyme targeted to PreC/C mRNA as potential therapy for hepatitis B, pCDNA3.1 (+) -Pre C/C vector was established for transcriping target mRNA. Synthesize a 10-23 DNA zyme corresponding to 2031 point of HBV PreC/C mRNA and observe its catalytic activity in vitro. Our results show that synthetic 10-23DNA zyme can efficiently cleave target mRNA in vitro, and its activity is dependent on the concentration of Mg 2+ ion.
- DNA zyme /
- hepatitis B virus

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