Abstract: To construct the stable transfective cell line with the eukaryotic expression vector for prealbumin cDNA.PA cDNA was obtained by RT-PCR of total RNA extracted from the human fetal liver.The sequence was inserted into pGEM-T Easy vector and then transfered into eukaryotic expression vector pCDNA 3 1 (+) .The recombination with forward insert were selected by restriction endonuclease digestion and confirmed correct by sequencing and then transfected into Hela cell line by using lipofectamine.The stable transfected cell lines were selected in medium containing geneticin (G418) .The full length prealbumin cDNA was inserted into the eukaryotic expression vector pCDNA3 1 (+) .The result of RT-PCR of total RNA exrtacted from the stable transfected cell lines showed that there exist the expression of PA cDNA in it.This work may pave the way for obtaining production of prealbumin in future.