Abstract: To evaluate the effect of the treatment of chronic hepatitis B with acupuncture combined with interferon-α (IFN-α) , 58 patients with chronic hepatitis B (with positive HBeAg and HBV DNA and elevated ALT) enrolled were randomized into acupuncture combined with IFN-α group (observed group) and IFN-α group (control group) .30 patients received acupuncture daily for 2 months and 5 million units IFN-α daily for the first 2 weeks then 3 times per week for 6 months.28 patients only received 5 million units IFN-α daily for the first 2 weeks then 3 times per week for 6 months.The efficacy and safety were evaluated with clinical, biochemical and virological parameters. After 2?6?12 months treatment HBV DNA?HBcAg seroconversion rate and ALT normalization rate in observed group were higher than in control group (p<0.01) .In conclusion, acupunctrue combined with IFNα tretment significantly enhances the response rate in chronic hepatitis B patients with persistent HBeAg.It can effectively enhance HBV DNA?HBeAg negative rate and ALT normalizstion rate, and enlarge the treatment range of IFN-α.It starts a new way to treat chronic hepatitis B with TCM combined with western medicine.