The effects of oxymatrine on apoptosis induced by TNF-α in human liver cell line L02
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摘要: 观察氧化苦参碱 (Oxy)对rhTNF -α诱导的L0 2肝细胞凋亡的影响。体外培养人L0 2肝细胞 ,采用H3-TdR法、台盼蓝染色及流式细胞仪观察Oxy对rhTNF -α引起的L0 2肝细胞的毒性作用影响。以rhTNF -α15ng/ml作用于细胞 2 4h ,细胞出现细胞核固缩 ,染色体凝集等凋亡样改变 ,台盼蓝染色阴性率在 95 %以上 ,应用流式细胞仪检测分析细胞周期 ,出现亚G1期峰 (凋亡AP峰 ) ,G2 -M期细胞明显减少。同时加入Oxy时 ,细胞生长率明显高于rhTNF -α处理组 ,并呈剂量相关性 ,在细胞周期图上未出现明显亚G1期峰。Oxy对rhTNF -α诱导的细胞凋亡有抑制作用Abstract: we investigate the effects of Oxy on apoptosis induced by rhTNF α in human liver cell line L02.Cell viability and proliferation was assayed by trypan blue stain and H 3 TdR co operalion assay.Flowcytometer was used for the cell cycle analysis using propidium iodide DNA binding.Apoptosis can be induced by rhTNF α in L02 cells.Evaluation of the PI DNA binding by flow cytometry showed abolition of G1, S, and G2/M phases and their replacement by a sub 2N AP peak of fragmented DNA.Characteristic apoptic condensations, ranging from single ball condensations to budding, and apoptic bodies were found in cells.Cells treated with Oxy+rhTNF α 15ng/ml partly retain the ability of proliferation, showing no significantly sub 2N Ap peak of fragmented DNA by flow cytometry.Oxy may have inhibitory activity on apoptosis of L02 cells induced by rhTNF α.
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Key words:
- Oxymatrine /
- Human Tumor Necrosis Factor α /
- apoptosis
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