PDF下载 ( 114 KB)
实验性肝纤维化时MMP-1、TIMP-1的表达与Ⅰ、Ⅲ型胶原含量变化的关系
Relativity of Expression of MMP-1、TIMP-1 and Variability of Type Ⅰ、Ⅲ Collagen During Experimental Liver Fibrosis
-
摘要: 为了观察实验性肝纤维化时MMP - 1、TIMP - 1的表达与Ⅰ、Ⅲ型胶原含量变化之间的关系 ,本文制备了免疫性大鼠肝纤维化模型 ,运用定量RT -PCR检测肝脏内MMP - 1、TIMP - 1的表达 ,通过免疫组化检测肝组织中Ⅰ、Ⅲ型胶原的含量。运用相关理论作统计学分析。结果发现在肝纤维化发生时 ,Ⅰ型胶原和Ⅲ型胶原含量的变化有显著相关性 ;MMP - 1的表达在肝纤维化组与正常对照组之间未见显著差异 ,与Ⅰ、Ⅲ型胶原的含量变化之间也未见有相关性 ;TIMP - 1的表达在肝纤维化发生时显著增高 ,与Ⅰ、Ⅲ型胶原的变化也有显著相关性。结果表明肝纤维化发生时MMP - 1的表达未见明显改变 ,而TIMP - 1的表达显著增高 ,且与Ⅰ、Ⅲ型胶原含量的增高呈显著相关 ,提示TIMP - 1的表达增高在肝纤维化的发生、发展中具有重要作用
-
关键词:
- 肝纤维化 /
- 基质金属蛋白酶-1 /
- 反义金属蛋白酶组织抑制因子-1 /
- Ⅰ型胶原 /
- Ⅲ型胶原
Abstract: To observe the relativity of expression of MMP-1、TIMP-1 and variability of type Ⅰ and Ⅲ collagen during experimental liver fibrosis, we made the rats liver fibrosis model by immune attack of human serum albumin (HSB) , and detected the expression of MMP-1 and TIMP-1 by quantitative RT-PCR, assayed the type I and type Ⅲ collagen by immunohistochemistry, then analyzed relativity of expression of MMP-1、TIMP-1 and variability of typeⅠ、Ⅲ collagen during liver fibrosis We found that the change of content of type Ⅰ and type Ⅲ collagen presented a high relativity (r=0 875, P<0 001) ;no difference was found between normal group and fibrosis group in the expression of MMP-1, and no relativity was found between the expression of MMP-1 and the change of content of type Ⅰ、type Ⅲ collagen;but we found the expression of TIMP-1 became significantly high after formation of liver fibrosis (P<0 01) , and it has the high relativity to the variability of type Ⅰand Ⅲ collagen So we got the conclusion that the expression of MMP-1 has no significant change during liver fibrosis, but that of TIMP-1 become obvious high, and there is a distinct relativity between the expression of TIMP-1 and the increasing of type Ⅰ、type Ⅲ collagen during liver fibrosis All of these suggest that increasing of the expression of TIMP-1 play a important role in the formation of liver fibrosis -
[]〔1〕FriedmanSL Thecellularbasisofhepaticfibrosis:mechanismsandtreat mentstrategies〔J〕 NEnglJMed , 1993, 32 3∶182 8 〔2〕DonC Rockey 各细胞成分在肝纤维化中的作用〔M〕 见王宝恩主编 肝病学新进展———基础与临床 , 1996年 1月 , 第一版 北京 :北京出版社 16 3 〔3〕MurawakiY , IkutaY , IdobeY , etal tissueinhibitorofmetalloproteinase-1intheliverofpatientswithchronicliverdisease〔J〕 JHepatol1997Jun ;2 6 (6 ) ∶12 13 〔4〕王宝恩 , 王志富 , 殷蔚荑 , 等 实验性免疫肝纤维化模型的研究〔J〕 中华医学杂志 , 1989, 6 9 (9) ∶5 0 3 〔5〕QuinnCO , ScottDK , BrinckerhoffCE , teal RatCollagenase Cloningaminoacidsequencecomparison , andparathyroidhermeneregulationinos teoblasticleas〔J〕 , JBiolChem , 1990 , 2 6 5∶2 2 342 []〔6〕QkadaA , GarnierJM , VicaireS , etal CloningoftheCDNAencodingrattissueinhibitorofmetnttoproteinasel (TIMP -1) , aminoacidcomparisonwithotherTIMPsandgeneexpressioninrattissue〔J〕 Gene 1994, 14 7∶30 1 〔7〕杨长青 , 胡国龄 , 谭德明 , 等 MMP -1表达质粒对大鼠肝纤维化的影响〔J〕 中华肝脏病杂志 , 1999, 7∶4 []〔8〕杨长青 , 胡国龄 , 谭德明 , 等 反义TIMP -1表达质粒对大鼠肝纤维化的影响〔J〕 中华内科杂志 , 2 0 0 0 , 39 (6 ) ∶40 1 []〔9〕OdazakiI, AraiM , WadaN , etal GnenexpressionofMMPsandTIMPsintheprocessofhepaticfibrosis〔J〕 Nipponrinsho , 1993, 5 1 (2 ) ∶42 8 []〔10〕BenyonRC , IredaleJP , GoddardS , etal Expressionoftissueinhititorofmetalloproteinase 1and 2isincreasedinfibrotichumanliver〔J〕 Gastroenterology, 1996 , 110 (3) ∶82 1 -
本文二维码
计量
- 文章访问数: 2763
- HTML全文浏览量: 45
- PDF下载量: 56
- 被引次数: 0
下载:
