维甲酸对贮脂细胞增殖和细胞内核酸含量的影响

高泽立; 李定国; 陆汉明; 顾晓红

维甲酸对贮脂细胞增殖和细胞内核酸含量的影响

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出版历程
- 出版日期: 2000-04-20

The Effect of Retinoic Acid on Ito Cell Proliferation and Content of Nucleic Acid

摘要

摘要: 为探讨维甲酸（Retinoicacid ,RA）对贮脂细胞的增殖和DNA、RNA含量影响 ,筛选抗肝纤维化有效药物。用原代培养的大鼠贮脂细胞 ,采用3H -TdR掺入实验 ,图象分析技术检测贮脂细胞增殖活性和DNA、RNA含量。结果维甲酸在 10 〔5～ 10 -4 mol/L可抑制贮脂细胞3H -TdR掺入 ;在 10 -4 mol/L可降低原代培养贮脂细胞的DNA、RNA含量 ,与对照组比较有显著性差异（P <0 .0 5 ）。表明维甲酸可抑制贮脂细胞3H -TdR掺入及降低原代培养贮脂细胞的DNA、RNA含量。RA可望成为有效的抗纤维化药物。
- 肝纤维化 /
- 维甲酸 /
- 贮脂细胞 /
- 细胞培养
Abstract: To clarify the mechanism of Retinoic acid (RA) against hepatofibrosis. 3H-TdR incorporationo test and Microspectrophotometer were used to analyse the proliferation of Ito cells and the DNA/RNA contents of Ito cells.The 3H-TdR incorporation in primary cultured Ito cells was suppressed by RA at 10 〔5 -1- -3 mol/L to the extent 50%-60%;DNA/RNA contents in primary cultured Ito cells treated with RA at 10 -4 mol/L were reduced as compared with the control.This suggests that vit A can inhibit Ito cell proliferation and prevent the transformation of Ito cells to my ofibroblasts.RA is expected to be an effective antihepatofibrotic agent in clinical practice.
- liver fibrosis /
- Retinoic acid (RA) Ito cell /
- cell culture /

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参考文献(6)

[]〔1〕RamadoriG .Thestellatecelloftheliver[J] VirchowsArchivBCelPath , 1991, 6 1 (3) ∶14 7

〔2〕高泽立 , 李定国 , 陆汉明贮脂细胞维甲酸含量及受体表达与肝纤维化 [J] 中华消化杂志 , 1993, 11 (5 ) ∶2 93

[]〔3〕高泽立 , 李定国 , 陆汉明维甲酸对贮脂细胞维甲酸受体和cAMP含量的影响 [J] 胃肠病学和肝病学杂志 , 1995 , 4 (1) ∶4

[]〔4〕YamaneM , TanakaY , MorwakiH , etal.InhibitoryeffectsofacyclicrinoidonhepaticfibrosisinCCl4-treatedrat[J] GastroenterolJap , 1990 , 2 5 (2 ) ∶2 2 3

[]〔5〕SeeifertA , HendriksH , RuiterG , etal.Beta -carotenedecreasestheseverityofCCl4-inducedhepaticinflammationandfibrosisinrat[J] Liver, 1995 , 15 (1) ∶1

[]〔6〕HashimotoY .Retinoicacidandunclearretinoicacidreceptor [J] CellStructureandFunction , 1991, 16 (2 ) ∶113

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