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The protective effect of oxymatrine on the fulminant hepatic failure in mice SIANG Xiao xing,
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摘要: 研究氧化苦参碱 （oxym atrine ,OM）对脂多糖 （LPS）加氨基半乳糖 （GalN）诱导的小鼠暴发型肝衰竭 （ful minanthepaticfailure ,FHF）的保护作用及其机理。实验分三组 :OM组 （10 0mgkg-1d-1× 3,ip） ,模型组及正常组。注射GaIN/LPS后 7.5小时 ,检测小鼠血清丙氨酸氨基转移酶 （ALT）和肿瘤坏死因子α（TNF -α）水平 ,同时取肝组织作HE染色和Fas及其配体 （FasL）的免疫组化检测。OM组小鼠ALT及TNF α水平明显低于模型组 （P <0 .0 1和 0 .0 5 ）。OM组肝损害程度及Fas、FasL表达强度均较模型组减轻 （P <0 .0 1）。OM可能通过抑制TNF -α活性及Fas、FasL表达 ,从而阻断LPS所致肝细胞凋亡及坏死Abstract: The experiment aimed to study the protective effect and its mechanism of oxymatrine (OM) on the fulminant hepatic failure (FHF) induced by injecting lipopolysaccharide (LPS) in galactosamine (GalN) sensitized mice.Thirty mice were divided into three groups:OM group (100mgkg -1 d -1 ×3, ip) , model group and normal group.The serum levels of alanine aminotransferase (ALT) and tumor necrosis factor alpha (TNF α) in all mice were determined at 7.5 hours after injecting GaIN/LPS.At the same time, the liver tissues were takan for HE staining and the immunohistochemical detection of Fas and its ligand (FasL) .Results:The serum levels of ALT and TNF α in mice of OM group were decreased significantly than that of model group (P<0.05 and 0.01, respectively) .Both the degrees of liver injury and expression of Fas and FasL in mice of OM group were reduced obviously than that of model group (P<0.01) .Conclution:It is possible that OM blocks the hepatocyte apoptosis and necrosis induced by LPS through inhibiting the activity of TNF α and the expression of Fas and FasL in liver tissue.
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Key words:
- Oxymatrine /
- Lipopolysaccharide /
- Fulminant hepatic failure /
- Fas antigen /
- Fas ligand
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