The effect of advanced glycation end products on biological activity of hepatic stellate cell

Objective To investigate the effect of advanced glycation end products on the biological activity of rat hepatic stellate cell (HSC) .Methods AGE-BSA was synthesized by incubating glucose with BSA in vitro.HSC-T6 was incubated with different concentrations of AGE-BSA.Messenger RNA type I and type Ⅲ precollagen, receptor of AGEs (RAGE) , TGF β1 and CTGF were determined by real-time PCR.Cell supernatant Type I and type Ⅲ precolagen, and TGF β1 were determined by ELISA.Results The expressions of mRNA type I and type Ⅲ precollagen, RAGE, TGF β1 and CTGF were increased after incubated with 100μg/ml AGE-BSA for 24 hours.The expression of mRNA level in cell supernatant increased with prolonged incubation time.Conclusion Glycation end products induce a time-dependant expression of precollagen and fibrogenic cytokines.Glycation end products may have a role in the development of liver fibrosis.