矿物中药硇砂提取液抑制肝癌的实验研究

To prepare a kind of Chinese traditional medicine extract liquid to substitute for absolute alcohol with low toxicity, good dissemination and led tumor necrosis more complete.Prepare Sal-Ammoniae extract liquid;in vitro we adopted CBRH-7919 liver cancer cell strain to conduct the cancer cell killing effect;in vivo rats loaded with CBRH-7919 cancer cell strain were used to appraise the anti-cancer effect by means of intra-tumor injection.Our experiment showed the Sal-Ammoniae extract liquid showed relative better cancer cell killing effect in vitro and cancer inhibiting effect in vivo.In vitro experiment the Sal-Ammoniae extract liquid showed the same or even better anti-cancer effect comparing with absolute alcohol and also it showed significant dose-effect relationship;In vivo experiment showed the Sal-Ammoniae extract liquid notably decreased the volume of the tumor, even led necrosis by intra-tumor injection, there was significant difference between experiment group and control group.The preliminary toxic experiment showed the extract has relative lower toxicity, similar to absolute alcohol, and relative lower side effects such as local stimulation etc.Conclusions: The Sal-Ammoniae extract liquid showed better tumor inhibiting effect in whole, better dissemination and more complete effect than absolute alcohol, so it is possible to be used as a topical remedy for liver cancer and other malignant solid tumors.