Role and mechanism of action of Sanhuang Xiexin Decoction in improving inflammatory injury of brain tissue in rats with hepatic encephalopathy

Ruixi ZHONG; Yuanqin DU; Xiyu XIAO; Wenxuan SONG; Yaobin NONG; Ronghuo ZHU; Jingjing HUANG

doi:10.3969/j.issn.1001-5256.2023.09.015

Volume 39 Issue 9

Sep. 2023

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Article Navigation > Journal of Clinical Hepatology > 2023 > 39(9): 2128-2136

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Role and mechanism of action of Sanhuang Xiexin Decoction in improving inflammatory injury of brain tissue in rats with hepatic encephalopathy

DOI: 10.3969/j.issn.1001-5256.2023.09.015

1.
Graduate School，Guangxi University of Chinese Medicine，Nanning 530299，China
2.
Department of Spleen，Stomach and Liver Diseases，The First Affiliated Hospital of Guangxi University of Chinese Medicine，Nanning 530299，China
3.
Guangxi Key Laboratory of Integrated Traditional Chinese and Western Medicine for High－incidence Infectious Diseases，Nanning 530299，China

Research funding:

Regional Program for National Natural Science Foundation of China (81760845);

General Program for Guangxi Natural Science Fundation (2021JJA141056);

Innovation Project of Guangxi Graduate Education (YCSW2021220);

Gui Sect Traditional Chinese Medicine Inheritance and Innovation Team (2022A001)

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Corresponding author: HUANG Jingjing， 55869563@qq.com （ORCID： 0000－0002－4936－0838）
Received Date: 2022-12-27
Accepted Date: 2023-03-20
Published Date: 2023-09-19

Abstract

Abstract

Objective To investigate the role and mechanism of action of Sanhuang Xiexin Decoction in improving inflammatory injury of brain tissue in rats with hepatic encephalopathy （HE） by establishing a rat model of HE. Methods A total of 30 Sprague－Dawley rats were randomly divided into normal group， model group， lactulose group （0.167 g/kg）， low－dose Sanhuang Xiexin Decoction group （4.37 g/kg crude drug）， and high－dose Sanhuang Xiexin Decoction group （8.74 g/kg crude drug）， with 6 rats in each group. The rats were given subcutaneous injection of 40% carbon tetrachloride oil solution （twice a week for 9 weeks， at a dose of 5 mL/kg each time in the first week， followed by 3 mL/kg each time） to establish a model of liver cirrhosis， and then they were given intraperitoneal injection of thioacetamide （300 mg/kg， once a day for 3 consecutive days） to establish a model of HE. After modeling， each treatment group was given the corresponding drug by gavage for 1 week. Morris water maze was used to observe the learning and memory abilities of rats after modeling and administration， and then the rats were sacrificed to collect blood， liver， and colon samples， with brain tissue collected on ice. HE staining was used to observe the pathological sections of liver and brain tissue； liver function， intestinal pH value， and blood ammonia were measured for all rats； ELISA was used to measure the serum levels of tumor necrosis factor－α （TNF－α）， interleukin－1β （IL－1β）， and interleukin－6 （IL－6）； the water content of brain tissue was calculated； quantitative real－time PCR was used to measure the mRNA expression levels of TNF－α， IL－1β， and IL－6， and Western blotting was used to measure the expression levels of nuclear factor－kappa B （NF－κB） p65 and phosphorylated NF－κB p65. A one－way analysis of variance was used for comparison of continuous data between multiple groups， and the least significant difference t－test or the Dunnett’s T3 method was used for further comparison between two groups. Results Compared with the model group， the low－and high－dose Sanhuang Xiexin Decoction groups had significant alleviation of the degeneration and necrosis of hepatocytes and brain cells and improvement in liver function in rats with HE （all P<0.05）， significant reductions in blood ammonia and the serum levels of TNF－α， IL－1β， and IL－6 （all P<0.05）， a relatively normal intestinal pH value （all P<0.05）， significant improvements in searching platform latency and number of platform crossings in the Morris water maze test （all P<0.05）， significant reductions in the water content of brain tissue and the mRNA expression levels of TNF－α， IL－1β， and IL－6 （all P<0.05）， and significantly inhibited expression levels of NF－κB p65 and its phosphorylated protein （all P<0.05）， and high－dose Sanhuang Xiexin Decoction tended to have a significantly better effect than low－dose Sanhuang Xiexin Decoction （all P<0.05）. Conclusion Sanhuang Xiexin Decoction can improve inflammatory injury of brain tissue in HE rats by effectively inhibiting the transcriptional level of inflammatory factors and NF－κB p65 phosphorylation in the brain of HE rats.
- Hepatic Encephalopathy,
- Xie Xin Tang,
- Treatment Outcome,
- Rats， Sprague－Dawley

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Role and mechanism of action of Sanhuang Xiexin Decoction in improving inflammatory injury of brain tissue in rats with hepatic encephalopathy

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Role and mechanism of action of Sanhuang Xiexin Decoction in improving inflammatory injury of brain tissue in rats with hepatic encephalopathy

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