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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 39 Issue 8
Aug.  2023
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Article Navigation >  Journal of Clinical Hepatology  > 2023  >  39(8): 1848-1856

Differentially expressed microRNAs in plasma exosomes from patients with chronic hepatitis B or hepatitis B virus-related acute-on-chronic liver failure: A bioinformatics analysis

DOI: 10.3969/j.issn.1001-5256.2023.08.013
  • 1.

    Qingdao Clinical Medical College of Nanjing Medical University, Qingdao, Shandong 266011, China

  • 2.

    Department of Infectious Diseases, Qingdao Municipal Hospital, Qingdao, Shandong 266011, China

  • 3.

    Clinical Research Center, Qingdao Municipal Hospital, Qingdao, Shandong 266071, China

  • 4.

    Department of Blood Purification, Qingdao Sixth People's Hospital, Qingdao, Shandong 266033, China

Research funding:

National Natural Science Foundation of China (82202416);

Special Fund for Artificial Liver of Beijing Public Welfare Foundation (RGGJJ-2021-030)

More Information
  • Corresponding author: LIU Sheng, lius123@163.com (ORCID: 0000-0002-9485-0211); XIN Yongning, xinyongning@163.com (ORCID: 0000-0002-3692-7655)
  • Received Date: 2022-12-07
  • Accepted Date: 2023-01-17
  • Published Date: 2023-08-20
    Abstract
  •   Objective  To investigate the differences in plasma exosomal microRNA (miRNA) expression profile between patients with chronic hepatitis B (CHB) and those with hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF), as well as their functions and biological processes, and to provide a reference for the clinical diagnosis of HBV-ACLF.  Methods  Six patients with CHB who were hospitalized in Department of Infectious Diseases, Qingdao Municipal Hospital, and six patients with HBV-ACLF who were treated in Blood Purification Center of Qingdao Sixth People's Hospital from October 2021 to June 2022 were enrolled. Illumina high-throughput sequencing was used to analyze the plasma exosomal miRNAs of these patients to obtain the differentially expressed miRNAs between the two groups. The differentially expressed miRNAs were screened, and a functional enrichment analysis was performed to identify the biological processes involving such miRNAs. The Mann-Whitney U rank sum test was used for comparison of continuous data between two groups, and the chi-square test was used for comparison of categorical data between groups.  Results  A total of 249 differentially expressed exosomal miRNAs were obtained according to the criteria of upregulation >2-fold or downregulation >2-fold, with P < 0.05, and compared with the CHB group, there were 126 upregulated miRNAs and 123 downregulated miRNAs. The bioinformatics analysis showed that these differentially expressed miRNAs were mainly involved in the biological processes such as gonadal development, regulation of protein stability, and cellular response to external stimuli, and they were closely associated with the signaling pathways such as hepatitis B, the role of proteoglycans in cancer, regulation of stem cell pluripotency, MAPK, Hippo, TNF, and lipid metabolism.  Conclusion  The differentially expressed miRNAs identified by Illumina high-throughput sequencing may be used as biomarkers for the early diagnosis and prognostic evaluation of HBV-ACLF.

     

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