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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 39 Issue 4
Apr.  2023
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An investigation of hepatitis D virus infection among patients with chronic hepatitis B virus infection in some regions of China

DOI: 10.3969/j.issn.1001-5256.2023.04.009
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  • Corresponding author: LIU Xueen, xueenliu@bjmu.edu.cn (ORCID: 0000-0002-6574-4989); ZHUANG Hui, zhuangbmu@126.com (ORCID: 0000-0001-9119-6325)
  • Received Date: 2022-11-06
  • Accepted Date: 2022-12-10
  • Published Date: 2023-04-20
  •   Objective  To investigate the prevalence of hepatitis D virus (HDV) infection among patients with chronic hepatitis B virus (HBV) infection in some regions of China.  Methods  Serum samples were collected from 3 131 patients with chronic HBV infection in 10 provinces, cities, and autonomous regions of China from March 2021 to June 2022, and anti-HDV IgG ELISA was used for the detection of all serum samples. Nested reverse transcription-polymerase chain reaction (nRT-PCR) was used to detect HDV RNA in anti-HDV IgG-positive samples, and the nRT-PCR amplification products of HDV RNA-positive samples were sequenced and analyzed to determine HDV genotype. The clinical features of anti-HDV IgG-positive patients were analyzed. The Mann-Whitney U rank sum test was used for comparison of continuous data between two groups, and the chi-square test or the Fisher's exact test was used for comparison of categorical data between two groups.  Results  The positive rate of anti-HDV IgG in the 3 131 patients with chronic HBV infection was 0.70% (22/3 131), and that in the patients with chronic HBV infection in Inner Mongolia Autonomous Region, Xinjiang Uygur Autonomous Region, Beijing, and Hunan Province was 1.81% (16/886), 0.88% (2/226), 0.28% (2/708), and 1.00% (2/200), respectively; the patients with chronic HBV infection in Inner Mongolia Autonomous Region had a significantly higher positive rate of anti-HDV IgG than those in Beijing (P=0.004), and there was no significant difference between the other regions (P > 0.05). Clinical features of the patients with chronic HBV infection in Inner Mongolia Autonomous Region showed that compared with the anti-HDV IgG-negative group, the anti-HDV IgG-positive group had a significantly higher proportion of patients with Mongol nationality (P=0.001), abnormal alanine aminotransferase (P=0.007), or antiviral treatment (P=0.029), as well as a significantly lower median HBV DNA level (P=0.030). A total of 19 HDV RNA-positive samples were identified, all of which had HDV genotype 1.  Conclusion  The prevalence rate of HDV varies greatly across different regions of China, with a higher prevalence rate of HDV in patients with chronic HBV infection from Inner Mongolia Autonomous Region. HDV genotype 1 is the predominant genotype in some provinces and cities of northern China.

     

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