中文｜English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 38 Issue 8
Aug.  2022
Turn off MathJax
Article Contents
Article Navigation >  Journal of Clinical Hepatology  > 2022  >  38(8): 1780-1783

Effect of Huatan Qushi Huoxue prescription on the ultrastructure of hepatocyte mitochondria in rats with nonalcoholic steatohepatitis

DOI: 10.3969/j.issn.1001-5256.2022.08.012

  • Department of Hepatology and Spleen-Stomach, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, China

Research funding:

General Project of National Natural Science Foundation of China (81473651);

Henan Province Traditional Chinese Medicine Scientific Research Special Project (2018JDZX005);

Henan Province Traditional Chinese Medicine Scientific Research Special Project (2019JDZX2051);

Science and technology project of Henan Province (202102310495);

Construction project of Traditional Chinese Medicine with Characteristic backbone discipline in Henan Province (STG-ZYXKY-2020024)

More Information
  • Corresponding author: ZHAO Wenxia, zhao-wenxia@163.com(ORCID: 0000-0002-9124-1111)
  • Received Date: 2021-12-29
  • Accepted Date: 2022-01-30
  • Published Date: 2022-08-20
    Abstract
  •   Objective  To investigate the effect of Huatan Qushi Huoxue prescription on the ultrastructure of hepatocyte mitochondria in a rat model of nonalcoholic steatohepatitis (NASH).  Methods  A total of 48 male Sprague-Dawley rats were randomly divided into blank group, model group, Yishanfu group, and Huatan Qushi Huoxue prescription group, with 12 rats in each group. The rats in the model group and the drug groups were administered and modeled since week 2; the rats in the blank group were given normal diet, and those in the other three groups were given high-fat diet. Based on dose conversion between human and animal, the equivalent dose of Huatan Qushi Huoxue prescription was 1.26 g/100 g body weight, and the equivalent dose of polyene phosphatidylcholine capsules (Yishanfu) was 0.014 18 g/100 g body weight. The rats in the model group were given 0.9% sodium chloride by gavage, those in the Yishanfu group were given polyene phosphatidylcholine suspension by gavage, and those in the traditional Chinese medicine group were given the granules of Huatan Qushi Huoxue prescription by gavage, once a day for 10 consecutive weeks. A transmission electron microscope was used to observe liver ultrastructure and perform a quantitative analysis. A one-way analysis of variance was used for comparison of continuous data between multiple groups; for further pairwise comparison, the least significant difference t-test was used for data with homogeneity of variance, and the Dunnett's T3 was used for data with heterogeneity of variance.  Results  The model group had a large number of lipid droplets accumulated in hepatocytes, changes in mitochondrial morphology and structure, and reductions in the number of mitochondria and endoplasmic reticulum. The Huatan Qushi Huoxue prescription group had a significant reduction in lipid droplets in hepatocytes and significant increases in the number of mitochondria and endoplasmic reticulum compared with the model group, with intact mitochondrial membrane and structure. The Yishanfu group had a reduction in lipid droplets in hepatocytes, an increase in the number of mitochondria, and a reduction in the number of endoplasmic reticulum, with relatively intact mitochondrial membrane and structure. The quantitative analysis showed that compared with the blank group, the model group had a significant increase in the area of lipid droplets and a significant reduction in mitochondria, with a significant difference in mitochondrial density between the two groups (all P < 0.01); after drug intervention, the Yishanfu group had a significant reduction in the area of lipid droplets and a significant increase in the number of mitochondria, with a significant difference in mitochondrial density between the Yishanfu group and the model group (all P < 0.01); compared with the Yishanfu group, the traditional Chinese medicine group had a significantly greater reduction in the area of lipid droplets and a significant increase in the number of mitochondria, with a significant difference in mitochondrial density between the two groups (all P < 0.05).  Conclusion  Huatan Qushi Huoxue prescription can improve lipid accumulation, increase mitochondrial density, and protect mitochondrial structure and function, with a better clinical effect than Yishanfu.

     

    • FullText(HTML)
  • loading
    • References(8)
  • [1]
    YOUNOSSI Z, ANSTEE QM, MARIETTI M, et al. Global burden of NAFLD and NASH: trends, predictions, risk factors and prevention[J]. Nat Rev Gastroenterol Hepatol, 2018, 15(1): 11-20. DOI: 10.1038/nrgastro.2017.109.
    [2]
    MANNE V, HANDA P, KOWDLEY KV. Pathophysiology of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis[J]. Clin Liver Dis, 2018, 22(1): 23-37. DOI: 10.1016/j.cld.2017.08.007.
    [3]
    ZHAO WX, ZHANG LH, CUI JJ. Research on Huatan Qushi Huoxue recipe on inflammation and fibrosis in pathological structure of rats with non-alcoholic steatohepatitis[J]. Chin Arch Tradit Chin Med, 2017, 35(9): 2229-2231. DOI: 10.13193/j.issn.1673-7717.2017.09.005.

    赵文霞, 张丽慧, 崔健娇. 化痰祛湿活血方对非酒精性脂肪性肝炎大鼠炎症损伤及纤维化的干预作用[J]. 中华中医药学刊, 2017, 35(9): 2229-2231. DOI: 10.13193/j.issn.1673-7717.2017.09.005.
    [4]
    ZHANG LH, ZHANG JB, ZHAO WX. Effect of Huatan Qushi Huoxue prescription on the key proteins of the ADPN/PI3K/Akt signaling pathway in rats with nonalcoholic steatohepatitis[J]. J Clin Hepatol, 2020, 36(4): 835-839. DOI: 10.3969/j.issn.1001-5256.2020.04.025.

    张丽慧, 张剑波, 赵文霞. 化痰祛湿活血方对非酒精性脂肪性肝炎大鼠模型ADPN/PI3K/AKT通路关键蛋白的影响[J]. 临床肝胆病杂志, 2020, 36(4): 835-839. DOI: 10.3969/j.issn.1001-5256.2020.04.025.
    [5]
    ZHAO WX, XU SC. Effect of Huatan Qushi Huoxue formula on expressions of oxidative stress related factors in rats with NASH[J]. Liaoning J Tradit Chin Med, 2017, 44(5): 1100-1102. DOI: 10.13192/j.issn.1000-1719.2017.05.065.

    赵文霞, 许顺畅. 化痰祛湿活血方对非酒精性脂肪性肝炎大鼠相关氧化应激因子的影响[J]. 辽宁中医杂志, 2017, 44(5): 1100-1102. DOI: 10.13192/j.issn.1000-1719.2017.05.065.
    [6]
    CAI TY, HUANG MZ, CHEN H, et al. Mechanism of action of mitochondrial disorders in nonalcoholic steatohepatitis[J]. J Clin Hepatol, 2021, 37(8): 1953-1956. DOI: 10.3969/j.issn.1001-5256.2021.08.045.

    蔡恬莹, 黄美州, 陈浩, 等. 线粒体障碍在非酒精性脂肪性肝炎中的作用机制[J]. 临床肝胆病杂志, 2021, 37(8): 1953-1956. DOI: 10.3969/j.issn.1001-5256.2021.08.045.
    [7]
    REN BY, YAO H, LU BJ. Mechanism of Zeming Hongshan granules in the treatment of non-alcohol-ic steatohepatitis through Sirt1/Foxa1 pathway: based on the theory of "spleen governs muscles"[J]. Chin J Gerontol, 2021, 41(24): 5607-5614. DOI: 10.3969/j.issn.1005-9202.2021.24.038.

    任柏樾, 姚辉, 卢秉久. 基于"脾主肌肉"理论探讨Sirt1/Foxa1通路介导泽明红山颗粒治疗非酒精性脂肪性肝炎的机制[J]. 中国老年学杂志, 2021, 41(24): 5607-5614. DOI: 10.3969/j.issn.1005-9202.2021.24.038.
    [8]
    ZHAO WX, LIU QZ, LIU JY, et al. Clinical observation of Huatan Lishi Huoxue formula for 96 patients with non-alcoholic steatohepatitis[J]. J Tradit Chin Med, 2012, 53(12): 1025-1027. DOI: 10.13288/j.11-2166/r.2012.12.002.

    赵文霞, 刘全忠, 刘君颖, 等. 化痰利湿活血方加减治疗非酒精性脂肪性肝炎患者96例临床观察[J]. 中医杂志, 2012, 53(12): 1025-1027. DOI: 10.13288/j.11-2166/r.2012.12.002.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(4)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (435) PDF downloads(47) Cited by()
    Proportional views
    Related
        

    The first journal specializing in hepatobiliary and pancreatic diseases in China

    Supervisor：Ministry of Education of the People's Republic of China

    Sponsor：Jilin University

    Academic Support: Chinese Society of Hepatology，Chinese Medical Association

    Address: 461 Xinjiang Road, Changchun

    Submit：0431-88782044

    Peer review：0431-88783542

    Email：lcgdb@vip.163.com

    About Journal

    Submission Guideline

    Journal Online

    Hepatobiliary College

    Guidelines

    YesterdayIP[]YesterdayPV[]TodayIP[]TodayPV[]Online[]

    Website Design © 2020 Editorial Board of Journal of Clinical Hepatology 吉ICP备10000617号-1 Supported by: Beijing Renhe Information Technology Co. Ltd  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return