Protective effect of tanshinone I in a mouse model of hepatic ischemia-reperfusion injury

Xiaokang YI; Yichao DU; Baolin QIAN; Zhiwei HUANG; Qiu LI; Wenguang FU; Jian WEN

doi:10.3969/j.issn.1001-5256.2021.01.021

Volume 37 Issue 1

Jan. 2021

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Protective effect of tanshinone I in a mouse model of hepatic ischemia-reperfusion injury

DOI: 10.3969/j.issn.1001-5256.2021.01.021

Xiaokang YI^{a, b},
Yichao DU^{a, b},
Baolin QIAN^b,
Zhiwei HUANG^b,
Qiu LI^b,
Wenguang FU^{a, b},
Jian WEN^{a, b
,
,}

a.
Academician (Expert) Workstation of Sichuan Province, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China
b.
Department of Hepatobiliary Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China

Received Date: 2020-07-23
Accepted Date: 2020-08-24
Published Date: 2021-01-20

Abstract

Abstract

Objective To investigate the protective effect of tanshinone I (T-I) on hepatic ischemia-reperfusion injury (HIRI) in mice. Methods A total of 36 C57BL/6J mice were randomly divided into sham-operation group, ischemia-reperfusion (IR) group, IR+T-I (5 mg/kg) group, IR+T-I (10 mg/kg) group, IR+T-I (20 mg/kg) group, and IR+T-I (40 mg/kg) group, with 6 mice in each group. Each group was given intraperitoneal injection. The mice in the sham-operation group and the IR group were injected with an equal volume of the solvent olive oil; the mice in the IR+T-I groups were administered once a day for 7 consecutive days, a model of 70% HIRI was established at 2 hours after the last administration, and serum and liver samples were collected after 6 hours of reperfusion. Related kits were used to measure the serum level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the content of superoxide dismutase (SOD), malondialdehyde (MDA), caspase-3, and reduced glutathione (GSH) in liver tissue; HE staining was used to observe liver histopathology; the TUNEL method was used to measure the level of hepatocyte apoptosis; immunohistochemistry was used to measure the protein expression of caspase-3 and heme oxygenase-1 (HO-1). A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. Results Compared with the IR group, the IR+T-I (20mg/kg) group had significant reductions in the serum levels of ALT (192.48±23.67 U/L vs 336.90±41.52 U/L, P < 0.01) and AST (123.19±9.16 U/L vs 206.90±18.81 U/L, P < 0.01), and thus 20 mg/kg was determined as the optimal concentration. Compared with the IR group, the IR+T-I (20 mg/kg) group had significant reductions in MDA (1.34±0.21 μmol/mg vs 3.48±0.95 μmol/mg, P < 0.05) and caspase-3 (0.69±0.97 μmol/mg vs 1.04±0.35 μmol/mg, P < 0.05) and significant increases in SOD (274.47±30.53 U/mg vs 160.29±27.37 U/mg, P < 0.05) and GSH (2.12±0.27 μmol/mg vs 1.03±0.42 μmol/mg, P < 0.05). HE staining showed that the IR group had disordered structure of hepatic lobules and focal or extensive degeneration and necrosis of hepatocytes; compared with the IR group, the IR+T-I (20 mg/kg) group had a reduction in the area of hepatocyte necrosis and a basically complete structure of the liver. Immunohistochemistry showed that compared with the IR group, the IR+T-I (20 mg/kg) group had significant reductions in the number of apoptotic hepatocytes and the protein expression of caspase-3 and a significant increase in the protein expression of HO-1. Conclusion T-I exerts a protective effect against HIRI in mice by inhibiting liver oxidative stress response and hepatocyte apoptosis.
- Reperfusion Injury,
- Oxidative Stress,
- TANSHINONE

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Protective effect of tanshinone I in a mouse model of hepatic ischemia-reperfusion injury

DOI: 10.3969/j.issn.1001-5256.2021.01.021

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