中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 36 Issue 8
Aug.  2020
Turn off MathJax
Article Contents

Role of serum hepatitis B virus RNA quantification in guiding antiviral therapy for chronic hepatitis B

DOI: 10.3969/j.issn.1001-5256.2020.08.035
Research funding:

 

  • Published Date: 2020-08-20
  • Chronic hepatitis B virus(HBV) infection and end-stage liver disease caused by such infection pose a serious threat to the health of Chinese citizens.The presence of HBV ccc DNA is the main reason for the difficulties in the treatment of chronic hepatitis B and recurrence after drug withdrawal.HBV RNA,as a new serum marker,is produced by ccc DNA transcription,and theoretically,it can reflect the level and transcriptional activity of ccc DNA in hepatocytes.This article mainly introduces the formation of serum HBV RNA and analyzes its significance in evaluating the activity or status of ccc DNA in liver tissue and predicting patients' response during antiviral therapy and after drug withdrawal.Moreover,it is pointed out that further large-scale clinical studies are needed to verify and improve the clinical significance of serum HBV RNA measurement,so as to further optimize the regimens of antiviral therapy and guide the precision treatment of patients.

     

  • loading
  • [1] URBAN S,SCHULZE A,DANDRI M,et al. The replication cycle of hepatitis B virus[J]. J Hepatol,2010,52(2):282-284.
    [2] WANG J,SHEN T,HUANG X,et al. Serum hepatitis B virus RNA is encapsidated pregenome RNA that may be associated with persistence of viral infection and rebound[J]. J Hepatol,2016,65(4):700-710.
    [3] BLOCK TM,GUO H,GUO JT. Molecular virology of hepatitis B virus for clinicians[J]. Clin Liver Dis,2007,11(4):685-706.
    [4] PRANGE R. Host factors involved in hepatitis B virus maturation,assembly,and egress[J]. Med Microbiol Immunol,2012,201(4):449-461.
    [5] REGEV A,BERHO M,JEFFERS LJ,et al. Sampling error and intraobserver variation in liver biopsy in patients with chronic HCV infection[J]. Am J Gastroenterol,2002,97(10):2614-2618.
    [6] ROUSSELET MC,MICHALAK S,DUPRF,et al. Sources of variability in histological scoring of chronic viral hepatitis[J].Hepatology,2005,41(2):257-264.
    [7] SORIANO V,BARREIRO P,BENITEZ L,et al. New antivirals for the treatment of chronic hepatitis B[J]. Expert Opin Investig Drugs,2017,26(7):843-851.
    [8] WANG J,DU M,HUANG H,et al. Reply to:“Serum HBV pgRNA as a clinical marker for cccDNA activity”:Consistent loss of serum HBV RNA might predict the “para-functional cure”of chronic hepatitis B[J]. J Hepatol,2017,66(2):462-463.
    [9] WANG J,YU Y,LI G,et al. Relationship between serum HBV-RNA levels and intrahepatic viral as well as histologic activity markers in entecavir-treated patients[J]. J Hepatol,2018,68(1):16-24.
    [10] GIERSCH K,ALLWEISS L,VOLZ T,et al. Serum HBV pgRNA as a clinical marker for cccDNA activity[J]. J Hepatol,2017,66(2):460-462.
    [11] ALLWEISS L,LTGEHETMANN M,VOLZ T,et al. 42 pegylated-interferon-alpha alone or in combination with entecavir restores ISG responsiveness and reduces intrahepatic viral loads and antigenemia in hepatitis B virus infected humanized mice[J]. 2012,56(Suppl 2):s18-s19.
    [12] BELLONI L,ALLWEISS L,GUERRIERI F,et al. IFN-αinhibits HBV transcription and replication in cell culture and in humanized mice by targeting the epigenetic regulation of the nuclear cccDNA minichromosome[J]. J Clin Invest,2012,122(2):529-537.
    [13] WIELAND SF,EUSTAQUIO A,WHITTEN-BAUER C,et al.Interferon prevents formation of replication-competent hepatitis B virus RNA-containing nucleocapsids[J]. Proc Natl Acad Sci U S A,2005,102(28):9913-9917.
    [14] HUANG YW,TAKAHASHI S,TSUGE M,et al. On-treatment low serum HBV RNA level predicts initial virological response in chronic hepatitis B patients receiving nucleoside analogue therapy[J]. Antivir Ther,2015,20(4):369-375.
    [15] LIMOTHAI U,CHUAYPEN N,POOVORAWAN K,et al. Baseline and kinetics of serum hepatitis B virus RNA predict response to pegylated interferon-based therapy in patients with hepatitis B e antigen-negative chronic hepatitis B[J]. J Viral Hepat,2019,26(12):1481-1488.
    [16] van BMMEL F,BARTENS A,MYSICKOVA A,et al. Serum hepatitis B virus RNA levels as an early predictor of hepatitis B envelope antigen seroconversion during treatment with polymerase inhibitors[J]. Hepatology,2015,61(1):66-76.
    [17] LUO H,TAN N,KANG Q,et al. Hepatitis B virus pregenomic RNA status can reveal the long-term prognoses of chronic hepatitis B patients treated with nucleos(t)ide analogues[J].J Viral Hepat,2020,27(3):323-328.
    [18] JANSEN L,KOOTSTRA NA,van DORT KA,et al. Hepatitis B virus pregenomic RNA is present in virions in plasma and is associated with a response to pegylated interferon alfa-2a and nucleos(t)ide analogues[J]. J Infect Dis,2016,213(2):224-232.
    [19] van BMMEL F,van BMMEL A,KRAUEL A,et al. Serum HBV RNA as a predictor of peginterferon alfa-2a response in patients with HBe Ag-positive chronic hepatitis B[J]. J Infect Dis,2018,218(7):1066-1074.
    [20] JIA W,ZHU MQ,QI X,et al. Serum hepatitis B virus RNA levels as a predictor of HBe Ag seroconversion during treatment with peginterferon alfa-2a[J]. Virol J,2019,16(1):61.
    [21] TSUGE M,MURAKAMI E,IMAMURA M,et al. Serum HBV RNA and HBe Ag are useful markers for the safe discontinuation of nucleotide analogue treatments in chronic hepatitis B patients[J]. J Gastroenterol,2013,48(10):1188-1204.
    [22] FAN R,ZHOU B,XU M,et al. Association between negative results from tests for HBV DNA and RNA and durability of response after discontinuation of nucles(t)ide analogue therapy[J]. Clin Gastroenterol Hepatol,2020,18(3):719-727.
    [23] ZHANG K,LIU Y,CHEN R,et al. Antigenicity reduction contributes mostly to poor detectability of HBs Ag by hepatitis B virus(HBV)S-gene mutants isolated from individuals with occult HBV infection[J]. J Med Virol,2018,90(2):263-270.
    [24] KIM GA,LIM YS,AN J,et al. HBs Ag seroclearance after nucleoside analogue therapy in patients with chronic hepatitis B:Clinical outcomes and durability[J]. Gut,2014,63(8):1325-1332.
    [25] WANG J,CHEN X,WU Y,et al. Serum HBV RNA is a potential predictor of hepatitis B surface antigen reversion[J]. Hepatol Commun,2018,2(10):1168-1171.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (817) PDF downloads(237) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return