中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 36 Issue 3
Mar.  2020
Turn off MathJax
Article Contents

Regulatory effect of water extract of Jianpi Xiaoji prescription on autophagy of human liver cancer SMMC7721 cells

DOI: 10.3969/j.issn.1001-5256.2020.03.023
Research funding:

 

  • Received Date: 2019-12-06
  • Published Date: 2020-03-20
  • Objective To investigate the anti-liver cancer mechanism of water extract of Jianpi Xiaoji prescription and its effect on cell autophagy flow and autophagy-related proteins. Methods SMMC7721 cells were treated with water extract of Jianpi Xiaoji prescription.CCK-8 assay was used to measure cell proliferation; flow cytometry was used to measure cell apoptosis rate; Western blot was used to measure the expression of the autophagy-related proteins Beclin1,LC3,and P62; the formation of autophagosomes and the fusion of autophagosomes and lysosomes were monitored. The independent samples t-test was used for comparison of continuous data between two groups; a one-way analysis of variance was used for comparison between multiple groups,and the least significant difference t-test was used for further comparison between two groups. Results The CCK-8 assay showed that cell proliferation was significantly inhibited at 24,48,72,and 96 hours after the drug was added,and there was a significant difference between the drug treatment group and the control group( t =28. 458,81. 093,85. 328,and 100. 158,all P < 0. 001). Flow cytometry showed that compared with the control group,the drug treatment group had a significant increase in the apoptosis of liver cancer SMMC7721 cells( t =-42. 629,P < 0. 001). Western Blot showed that compared with the control group,the drug treatment group had significantly downregulated expression of Beclin1 and significantly upregulated expression of the autophagy markers LC3-Ⅱ and P62. The flow of autophagosomes to autolysosomes was blocked in the drug treatment group,and there was a significant difference between the drug treatment group and the control group( F = 31. 155,P < 0. 001). Conclusion The water extract of Jianpi Xiaoji prescription can inhibit the proliferation of human liver cancer SMMC-7721 cells and promote apoptosis; meanwhile,it can upregulate the expression of Beclin1,downregulate the expression of LC3 and P62,and block the formation of autophagy flux.

     

  • loading
  • [1] BRAY F,FERLAY J,SOERJOMATARAM I,et al. Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,2018,68(6):394-424.
    [2] VILLANUEVA A. Hepatocellular carcinoma[J]. New Engl J Med,2019,380(15):1450-1462.
    [3] SPARKMAN J,HERNAEZ R,EL-SERAG HB. Traditional Chinese Medicine to the rescue of allopathic medicine in the coadjuvant treatment of hepatocellular carcinoma[J]. Transl Gastroenterol Hepatol,2018,3:97.
    [4] HUANG ZF. Jianpi Xiaoji Tang[J]. Guangxi J Tradit Chin Med,2004,27(4):32.(in Chinese)黄智芬.健脾消积汤[J].广西中医药,2004,27(4):32.
    [5] HAUNG ZF,LI HZ,ZHANG ZJ,et al. Effect of Jianpixiaoji decoction on the quality of life of patients with advanced primary liver cancer[J]. Chin J Integrated Tradit West Med Digest,2010,18(1):47-48,50.(in Chinese)黄智芬,黎汉忠,张作军,等.健脾消积汤对晚期原发性肝癌患者生存质量的影响[J].中国中西医结合消化杂志,2010,18(1):47-48,50.
    [6] TIAN H,ZHU JY,MO XL,et al. Inhibitory effect of Jianpi Xiaoji decoction containing plasma on hepatocellular carcinoma HepG2 cells and its mechanism[J]. Guangxi Med J,2017,39(7):1025-1028.(in Chinese)田昊,诸佳瑜,莫晓丽,等.健脾消积汤含药血浆对肝细胞癌HepG2细胞的抑制作用及其机制[J].广西医学,2017,39(7):1025-1028.
    [7] KUBISCH J,TUREI D,FOLDVARI-NAGY L,et al. Complex regulation of autophagy in cancer-Integrated approaches to discover the networks that hold a double-edged sword[J].Semin Cancer Biol,2013,23(4):252-261.
    [8] CUI B,YU JM. Autophagy:A new pathway for traditional Chinese medicine[J]. J Asian Nat Prod Res,2018,20(1):14-26.
    [9] ZHANG Z,GAO WH,TIAN XF,et al. Effect of Yiqi Huayu Jiefang on SMCC-7721 autophagy flow in human hepatoma cells based on PI3K/m TOR pathway[J]. Chin J Inf Tradit Chin Med,2019,26(10):45-49.(in Chinese)张振,郜文辉,田雪飞,等.基于PI3K/m TOR通路益气化瘀解毒方对人肝癌细胞SMCC-7721自噬流的影响[J].中国中医药信息杂志,2019,26(10):45-49.
    [10] LU K,WANG J,SUN ZW. Silica nanopartides induce autophagy dysfunction in HepG2 cells[J]. J Health Toxicol,2017,31(4):267-272.(in Chinese)鲁可,王继,孙志伟.纳米Si O2引起肝细胞自噬功能紊乱的研究[J].毒理学杂志,2017,31(4):267-272.
    [11] JIANG X,HUANG Y,LIANG X,et al. Metastatic prostate cancer-associated P62 inhibits autophagy flux and promotes epithelial to mesenchymal transition by sustaining the level of HDAC6[J]. Prostate,2018,78(6):426-434.
    [12] METZ P,CHIRAMEL A,CHATEL-CHAIX L,et al. Dengue virus inhibition of autophagic flux and dependency of viral replication on proteasomal degradation of the autophagy receptor p62[J]. J Virol,2015,89(15):8026-8041.
    [13] TURCO E,WITT M,ABERT C,et al. FIP200 claw domain binding to p62 promotes autophagosome formation at ubiquitin condensates[J]. Mol Cell,2019,74(2):330-346. e11.
    [14] SETO S,TSUJIMURA K,HORII T,et al. Autophagy adaptor protein p62/SQSTM1 and autophagy-related gene Atg5 mediate autophagosome formation in response to Mycobacterium tuberculosis infection in dendritic cells[J]. PLo S One,2013,8(12):e86017.
    [15] LOOS B,KLIONSKY DJ,DU TOIT A. On the relevance of precision autophagy flux control in vivo-Points of departure for clinical translation[J]. Autophagy,2019.[Epub ahead of print]
    [16] MA RD,ZHOU GJ,QU M,et al. Corticosterone induces neurotoxicity in PC12 cells via disrupting autophagy flux mediated by AMPK/m TOR signaling[J]. CNS Neurosci Ther,2019,26(2):167-176.
    [17] SONG X,LIU L,CHANG M,et al. NEO212 induces mitochondrial apoptosis and impairs autophagy flux in ovarian cancer[J]. J Exp Clin Cancer Res,2019,38(1):239.
    [18] WANG H,DUAN PW,FU J,et al. Experimental study on the regulation of autophagy in reversing tumor drug resistance by Chinese herbal medicine[J]. Guid J Tradit Chin Med Pharm2019,25(13):53-56.(in Chinese)王浩,段佩雯,伏杰,等.中药单体调控自噬逆转肿瘤耐药的实验研究现状[J].中医药导报,2019,25(13):53-56.
    [19] LI J,LI X,GAO YY,et al. Research progress of controlling cell autophagy against tumor by traditional Chinese medicine[J]. J Liaoning Univ Tradit Chin Med,2019,6:8-11.(in Chinese)李冀,李想,高彦宇,等.中药调控细胞自噬抗肿瘤研究进展[J].辽宁中医药大学学报,2019,6:8-11.
    [20] WANG MQ,LI YP,LIU LY,et al. Role and mechanism of autophagy in development and progression of hepatocellular carcinoma and regulation of immunotherapy[J]. J Clin Hepatol,2019,35(5):1129-1134.(in Chinese)王沐淇,李亚萍,刘拉羊,等.自噬在肝细胞癌发生发展和免疫治疗调控中的作用及其机制[J].临床肝胆病杂志,2019,35(5):1129-1134.
    [21] DU Y,JI X. Bcl-2 down-regulation by small interfering RNA induces Beclin1-dependent autophagy in human SGC-7901cells[J]. Cell Biol Int,2014,38(10):1155-1162.
    [22] GUAN Y,LI Y,ZHAO G,et al. HMGB1 promotes the starvation-induced autophagic degradation of alpha-synuclein in SH-SY5Y cells Atg 5-dependently[J]. Life Sci,2018,202:1-10.
    [23] LISIAK N,TOTON E,RYBCZYNSKA M. Autophagy as a potential therapeutic target in breast cancer treatment[J]. Curr Cancer Drug Targets,2018,18(7):629-639.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (897) PDF downloads(205) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return