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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 9
Sep.  2018
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Article Contents

Association between single nucleotide polymorphism of low-density lipoprotein receptor and nonalcoholic fatty liver disease

DOI: 10.3969/j.issn.1001-5256.2018.09.021
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  • Received Date: 2018-03-28
  • Published Date: 2018-09-20
  • Objective To investigate the association between gene polymorphisms of low-density lipoprotein receptor (LDL-R) and nonalcoholic fatty liver disease (NAFLD) and the effect of LDL-R gene polymorphisms on serum lipid levels in the Chinese Han population.Methods A total of 216 NAFLD patients who visited Qingdao Municipal Hospital from October 2015 to July 2017 and 135 healthy subjects were enrolled. PCR and mass spectrometry were used to determine the genotypes of the rs1433099 and rs2738464 loci of LDL-R. The biochemical methods were used to measure the changes in blood lipid levels and liver enzymes. The Hardy-Weinberg (H-W) equilibrium was used to test the group representativeness of samples, and the chi-square test was used to test whether the distribution of genotypes was in accordance with the H-W equilibrium. The direct counting method was used to determine genotype and allele frequencies; the chi-square test or the Fisher's exact test was used for comparison of categorical data between groups; the t-test and wilcoxon rank sum test were used for comparison of continuous data between groups; the association of rs1433099 and rs2738464 polymorphisms with the relative risk of NAFLD was expressed as odds ratio (OR) and its 95% confidence interval (CI) , and the binary logistic regression model was used for calculation. Results There was no significant difference between the NAFLD group and the healthy control group in allele and genotype frequencies of LDL-R rs1433099 and rs2738464 (all P > 0. 05) . There were also no significant differences between the two groups in blood lipid levels, liver enzymes, and body mass index (BMI) (all P > 0. 05) . In the NAFLD group, the healthy control group, and all subjects, there were no significant differences in general clinical features, including blood lipid levels, BMI, blood pressure, and liver enzymes, between the subjects carrying rs1433099 T and rs2738464 G and those who did not carry such alleles (all P > 0. 05) . Conclusion The polymorphisms of LDL-R rs1433099 and rs2738464 do not affect blood lipid levels. This study demonstrates for the first time that the polymorphisms of LDL-R rs1433099 and rs2738464 are not associated with the risk of NAFLD in the Chinese Han population.

     

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