Clinical effect of Pien Tze Huang in treatment of guinea pigs with cholecystitis induced by lithocholic acid given by gavage

Objective To investigate the clinical effect of traditional Chinese medicine Pien Tze Huang in the treatment of guinea pigs with cholecystitis.Methods A total of 80 guinea pigs (40 males and 40 females) were randomly divided into blank control group (10 guinea pigs treated with PBS at the same dose as that in model group) , cholecystitis model group (20 guinea pigs treated with lithocholic acid given by gavage) , Pien Tze Huang group (30 guinea pigs treated with Pien Tze Huang by gavage in addition to the treatment in the cholecystitis model group) , and ursodeoxycholic acid group (20 guinea pigs treated with ursodeoxycholic acid in addition to the treatment in the cholecystitis model group) .All groups were observed in terms of symptoms, pathological damage of the gallbladder, and liver function [the levels of alanine aminotransferase (ALT) , aspartate aminotransferase (AST) , total bilirubin (TBil) , direct bilirubin (DBil) , indirect bilirubin (IBil) , and total bile acid (TBA) ].The two-independent-samples t test was used for comparison of continuous data between groups;a one-way analysis of variance was used for comparison between multiple groups and the least significant difference t-test was used for comparison between any two groups.P<0.05 was considered statistically significant.Results Compared with the model group, the Pien Tze Huang group had significantly better conditions with smooth hair, a good mental state, and good food consumption, and no guinea pigs died after 15 days.As was shown by ultrasound and rheography, compared with the model group, the Pien Tze Huang group had basically normal morphology of the gallbladder and significant reductions in sagittal diameter of the gallbladder (14.92±1.36 mm vs 16.51±0.65 mm, P<0.05) , transverse diameter of the gallbladder (21.25±1.61 mm vs 23.93±0.84 mm, P<0.05) , and gallbladder wall thickness (0.75± 0.049 mm vs 0.90±0.03 mm, P<0.05) .HE staining showed that Pien Tze Huang significantly improved the pathological damage of the gallbladder.Compared with the model group, the Pien Tze Huang group had significant reductions in ALT (316.50±264.50 U/L vs684.71±87.73 U/L, P<0.05) , AST (88.78±16.69 U/L vs 180.2±84.40 U/L, P<0.05) , TBil (0.18±0.10) μmol/L vs 1.43 ±0.90 μmol/L, P<0.05) , DBil (0.06±0.05 μmol/L vs 0.23±0.07 μmol/L, P<0.05) , IBil (0.12±0.06 μmol/L vs 1.20±0.55μmol/L, P<0.05) , and TBA (42.40±40.57 μmol/L vs 271.42±148.94 μmol/L, P<0.05) .Conclusion Pien Tze Huang can significantly improve the symptoms, pathological damage of the gallbladder, and liver function in guinea pigs with cholecystitis.