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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 4
Apr.  2016
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Effect of PNPLA3 I148M mutation on expression of TGF- β1 in rat hepatic stellate cells

DOI: 10.3969/j.issn.1001-5256.2016.04.035
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  • Published Date: 2016-04-20
  • Objective To investigate the mechanism of action of PNPLA3 I148 M mutation in the development and progression of non- alcoholic fatty liver fibrosis. Methods The lentiviral vectors carrying the mutant or wild- type PNPLA3 I148 M gene were constructed and transfected into rat hepatic stellate( HSC- T6) cells. Quantitative real- time PCR was applied to measure the mRNA expression of transforming growth factor β1( TGF β1). The t- test was applied for statistical analysis. Results The lentiviral vectors carrying the mutant or wild- type PNPLA3 I148 M gene were successfully constructed and transfected into HSC- T6 cells,and a HSC- T6 cell line with stable expression of the mutant or wild- type PNPLA3 gene was established. Compared with the cell line carrying the wild- type gene,the cell line carrying the mutant gene showed significantly higher mRNA expression of TGF β1( 1. 25 ± 0. 15 vs 0. 48 ± 0. 07; t = 11. 826,P < 0. 001).Conclusion PNPLA3 I148 M mutation can increase the expression of TGF β1 in HSC- T6 cells,which provides a new cell model and new research ideas for investigating the role of PNPLA3 I148 M mutation in non- alcoholic fatty liver fibrosis.

     

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