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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 9
Sep.  2014
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Article Contents

Establishment and management of nonalcoholic fatty liver disease biobank

DOI: 10.3969/j.issn.1001-5256.2014.09.017
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  • Received Date: 2013-11-28
  • Published Date: 2014-09-20
  • Objective To investigate the collection and preservation of blood specimens from patients with nonalcoholic fatty liver disease ( NAFLD) and the establishment and information management of biobank. Methods Whole blood samples were collected from 1226 patients who were diagnosed with NAFLD based on B- mode ultrasound and blood tests from October 2009 to October 2013. Biochemical parameters were measured. Plasma and whole- blood genomic DNA was extracted from the samples, and the purity and concentration of DNA were determined. Specimens were preserved in a refrigerator (- 80℃) . An information management system for NAFLD biobank was established. Results Specimens of 1226 NAFLD patients, including those of 83 twins and 100 families, were collected. The success rate was100% for extraction of plasma and whole- blood genomic DNA. One hundred DNA samples were randomly selected for testing, and the results showed that the collected specimens met the requirements of following experiments. Conclusion The NAFLD Biobank has been successfully established in this study. It has the standard information management system and enables the quality control and information management of specimens, laying a solid foundation for further research on NAFLD.

     

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