中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 8
Aug.  2014
Turn off MathJax
Article Contents

Analysis of clinical significance of quantitative expression of VEGF mRNA and uPA mRNA in pancreatic cancer tissue

DOI: 10.3969/j.issn.1001-5256.2014.08.014
Research funding:

 

  • Received Date: 2014-05-16
  • Published Date: 2014-08-20
  • Objective To investigate the clinical significance of quantitative expression of vascular endothelial growth factor (VEGF) mRNA and urokinase- type plasminogen activator (uPA) mRNA in pancreatic cancer tissue.Methods A retrospective study was conducted on the complete data of 30 patients with a pathological diagnosis of duct adenocarcinoma who were selected from those treated by radical resection of pancreatic head carcinoma from January 2008 to December 2011.Real- time quantitative PCR was used to measure the quantitative expression of VEGF mRNA and uPA mRNA in the pancreatic cancer tissues of the 30 cases and the normal pancreatic tissues of 6 controls, and its relationship with clinicopathological factors was analyzed.Results The quantitative expression of VEGF mRNA and uPA mRNA was correlated with the histological differentiation and perineural invasion of pancreatic cancer.The quantitative expression of VEGF mRNA was significantly higher in patients with lymphatic metastasis than in those without lymphatic metastasis group (t = 20.007, P = 0.000) .The quantitative expression of uPA mRNA was significantly lower in the tumors with a diameter of ≤2 cm than in the tumors with a diameter of > 2 cm (t = 7.539, P = 0.000) .Patients with duodenal invasion had a significantly higher quantitative expression of uPA mRNA than those without duodenal invasion (t =- 2.089, P = 0.037) .The quantitative expression of uPA mRNA in stage III tumor tissues was significantly higher than that of uPA mRNA in stage I and II tumor tissues (t =- 9.450, P = 0.000) .There was a positive correlation between VEGF mRNA expression and uPA mRNA expression (r = 0.334, P = 0.000) .Conclusion The overexpression of VEGF mRNA and uPA mRNA in pancreatic cancer tissue may create an environment that enables the invasion by pancreatic cancer cells.

     

  • loading
  • [1]XU KC.Investigation of treatment strategy for advanced cancer according to treatment of pancreatic cancer[J].J Clin Hepatol, 2013, 29 (7) :484-488. (in Chinese) 徐克成.从胰腺癌治疗探讨进展期癌症治疗策略[J].临床肝胆病杂志, 2013, 29 (7) :484-488.
    [2] JEMAL A, SIEGEL R, XU J, et al.Cancer statistics, 2010[J].CA Cancer J Clin, 2010, 60 (5) :277-300.
    [3]LEUNG DW, CACHIANES G, KUANG WJ, et al.Vascular endothelial growth factor is a secreted angiogenic mitogen[J].Science, 1989, 246 (4935) :1306-1309.
    [4]ZORGETTO VA, SILVEIRA GG, OLIVEIRA-COSTA JP, et al.The relationship between lymphatic vascular density and vascular endothelial growth factor A (VEGF-A) expression with clinicalpathological features and survival in pancreaticadenocarcinomas[J].Diagn Pathol, 2013, 8:170.
    [5]WEIDNER N.Tumoral vasculary as a prognostic factor in cancer patients:the evendence continues to grow[J].J Pathol, 1998, 184 (2) :119-122.
    [6]ROGERS A, SMITH MJ, DOOLAN P, et al.Invasive markers identified by gene expression profiling in pancreatic cancer[J].Pancreatology, 2012, 12 (2) :130-140.
    [7]HILDENBRAND R, NIEDERGETHMANN M, MARX A, et al.Amplification of theurokinasetype plasm inogen activator receptor (uPAR) gene in ductal pancreatic carcinomas identifies a clinically highrisk group[J].Am J Pathol, 2009, 174 (6) :2246-2253.
    [8]DOBRILA-DINTINJANA R, VANIS N, DINTINJANA M, et al.Etiology and oncogenesis of pancreatic carcinoma[J].Coll Antropol, 2012, 36 (3) :1063-1067.
    [9]ASUTHKAR S, STEPANOVA V, LEBEDEVA T, et al.Multifunctional roles of urokinase plasminogen activator (uPA) in cancer stemness and chemoresistance of pancreatic cancer[J].Mol Biol Cell, 2013, 24 (17) :2620-2632.
    [10]SCHLAEPI JM, WOOD JM.Targeting vascular endothelial growth factor (VEGF) for anti-tumor therapy, by anti-VEGF neutralizing monoclonal antibodies or by VEGF receptor tyrosine-kinase inhibitors[J].Cancer Metastasis Res, 1999, 18 (4) :473-481.
    [11]NIETHAMMER AG, LUBENAU H, MIKUS G, et al.Doubleblind, placebo-controlled first in human study to investigate an oral vaccine aimed to elicit an immune reaction against the VEGFReceptor 2 in patients with stage IV and locally advanced pancreatic cancer[J].BMC Cancer, 2012, 12:361.
    [12]HBEL S, KOBURGER I, JOHN M, et al.Polyethylenimine/small interfering RNA-mediated knockdown of vascular endothelial growth factor in vivo exerts anti-tumor effects synergistically with Bevacizumab[J].J Gene Med, 2010, 12 (3) :287-300.
    [13]MARTIN LK, LI X, KLEIBER B, et al.VEGF remains an interesting target in advanced pancres cancer (APCA) :results of a multi-institutional phase II study of bevacizumab, gemicitabine, and infusional 5-fluorouracil in patients with APCA[J].Ann Oncol, 2012, 23 (11) :2812-2820.
    [14]HILEY CT, CHARD LS, GANGESWARAN R, et al.Vascular endothelial growth factor A promotes vaccinia virus entry into host cells via activation of the Akt pathway[J].J Virol, 2013, 87 (5) :2781-2790.
    [15]KINDLER HL, NIEDZWIECKI D, HOLLIS D, et al.Gemcitabine plus bevacizumab compared with gemcitabine plus placebo in patients with advanced pancreatic cancer:phaseⅢtrial of the Cancer and Leukemia Group B (CALGB 80303) [J].J Clin Oncol, 2010, 28 (22) :3617-3622.
    [16]VAN CUTSEM E, VERVENNE WL, BENNOUNA J, et al.PhaseⅢtrial of bevacizumab in combination with gemcitabine and erlotinib in patients with metastatic pancreatic cancer[J].J Clin Oncol, 2009, 27 (13) :2231-2237.
    [17]LU JD.Novel therapeutic strategies for advanced pancreatic cancer:targeting the epidermal growth factor and vascular endothelial growth factor pathways[J].China Oncol, 2009, 19 (8) :590-596. (in Chinese) 陆嘉德.晚期胰腺癌的分子靶向治疗[J].中国癌症杂志, 2009, 19 (8) :590-596.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2614) PDF downloads(524) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return