Expression and clinical significance of matrix metalloproteinase 7 and heparan sulfate proteoglycan 2 in pancreatic cancer

Jinfeng CHEN; LU Xiaoliu; Xiaoyun ZHANG; Xiping TANG

doi:10.3969/j.issn.1001-5256.2023.09.020

Volume 39 Issue 9

Sep. 2023

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Article Navigation > Journal of Clinical Hepatology > 2023 > 39(9): 2169-2174

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Expression and clinical significance of matrix metalloproteinase 7 and heparan sulfate proteoglycan 2 in pancreatic cancer

DOI: 10.3969/j.issn.1001-5256.2023.09.020

1.
School of Medical Oncology，Guangxi Medical University，Nanning 530021，China
2.
Endoscopy Center，Guangxi Medical University Cancer Hospital，Nanning 530021，China

Research funding:

Guangxi Natural Science Foundation (2020GXNSFAA297062);

Guangxi Medical and Health Appropriate Technology Development and Promotion Application Project (S2020103);

Scientific Research Project of Guangxi Administration of Traditional Chinese Medicine (GZZC2020235)

More Information

Corresponding author: TANG Xiping， tangxiping108@hotmail.com （ORCID： 0000－0003－0156－9429）
Received Date: 2022-12-15
Accepted Date: 2023-02-06
Published Date: 2023-09-19

Abstract

Abstract

Objective To investigate the expression and clinical significance of matrix metalloproteinase 7 （MMP7） and heparan sulfate proteoglycan 2 （HSPG2） in patients with pancreatic cancer. Methods Pancreatic cancer tissue samples， normal adjacent tissue samples， and serum samples were collected from 30 patients who were admitted to Guangxi Medical University Cancer Hospital from January 2018 to December 2020， and serum samples were collected from 30 healthy volunteers as controls. Immunohistochemistry was used to measure the protein expression levels of MMP7 and HSPG2 in pancreatic cancer tissue samples and normal adjacent tissue samples； ELISA was used to measure the expression levels of MMP7 and HSPG2 in the serum samples of the pancreatic cancer patients and the healthy controls； related clinical data were collected to analyze the association of the expression of MMP7 and HSPG2 with clinicopathological features， survival， and prognosis. The independent－samples t test was used for comparison of continuous data between two groups， and the chi－square test or the Fisher’s exact test was used for comparison of categorical data between two groups； the Spearman correlation analysis was used to investigate the correlation between MMP7 and HSPG2； the Kaplan－Meier method was used to plot survival curves， and the Log－rank test was used for survival analysis. Results Immunohistochemistry showed that the protein expression levels of MMP7 and HSPG2 in pancreatic cancer tissue were significantly higher than those in adjacent tissue （χ²=31.093 and 35.623， both P<0.05）. The patients with pancreatic cancer had significantly higher serum levels of MMP7 and HSPG2 than the healthy controls （t=20.174 and 32.600， both P<0.05）. The expression of MMP7 and HSPG2 was associated with tumor diameter and TNM stage （both P<0.05）. The Spearman correlation analysis showed a positive correlation between HSPG2 and MMP7 （r=0.539， P=0.002）. The patients with positive protein expression of MMP7 and HSPG2 had a significantly lower overall survival rate than those with negative expression （χ²=4.084 and 12.554， both P<0.05）. Conclusion MMP7 and HSPG2 are highly expressed in pancreatic cancer tissue and serum and are associated with tumor diameter and TNM stage. They may also be associated with the development and progression of pancreatic cancer， and therefore， they are expected to become potential markers for the diagnosis and prognostic evaluation of pancreatic cancer.
- Pancreatic Neoplasms,
- Matrix Metalloproteinase 7,
- Heparan Sulfate Proteoglycans

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References(17)

References

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Expression and clinical significance of matrix metalloproteinase 7 and heparan sulfate proteoglycan 2 in pancreatic cancer

DOI: 10.3969/j.issn.1001-5256.2023.09.020

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Expression and clinical significance of matrix metalloproteinase 7 and heparan sulfate proteoglycan 2 in pancreatic cancer

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