中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 35 Issue 10
Oct.  2019
Turn off MathJax
Article Contents

Clinical application of covalently closed circular DNA detection techniques

DOI: 10.3969/j.issn.1001-5256.2019.10.002
Research funding:

 

  • Received Date: 2019-08-01
  • Published Date: 2019-10-20
  • Covalently closed circular DNA(cccDNA) is the original template for hepatitis B virus(HBV) replication and is the basis of persistent HBV infection. Only when HBV cccDNA is completely eliminated can HBV be eradicated. Therefore,the detection of HBV cccDNA plays an important role in evaluating the antiviral outcome of chronic hepatitis B,assessing HBV clearance,clarifying occult HBV infection,studying the pathogenesis of hepatitis B,and developing new drugs,and HBV cccDNA detection techniques with high sensitivity and specificity are the basis for clinical application. With reference to related reports in literature and our own research work,this article reviews the development and clinical application of HBV cccDNA detection methods in recent years.

     

  • loading
  • [1] SIMMONDS P,BUKH J,COMBET C,et al. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes[J]. Hepatology,2005,42(4):962-973.
    [2] COX AL,NETSKI DM,MOSBRUGER T,et al. Prospective evaluation of community-acquired acute-phase hepatitis C virus infection[J]. Clin Infect Dis,2005,40(7):951-958.
    [3] LUCIFORA J,PROTZER U. Attacking hepatitis B virus cccDNA-The holy grail to hepatitis B cure[J]. J Hepatol,2016,64(1 Suppl):s41-s48.
    [4] SUN D,NASSAL M. Stable HepG2-and Huh7-based human hepatoma cell lines for efficient regulated expression of infectious hepatitis B virus[J]. J Hepatol,2006,45(5):636-645.
    [5] WERLE-LAPOSTOLLE B,BOWDEN S,LOCARNINI S,et al.Persistence of cccDNA during the natural history of chronic hepatitis B and decline during adefovir dipivoxil therapy[J].Gastroenterology,2004,126(7):1750-1758.
    [6] BOWDEN S,JACKSON K,LITTLEJOHN M,et al. Quantification of HBV covalently closed circular DNA from liver tissue by real-time PCR[J]. Methods Mol Med,2004,95:41-50.
    [7] SINGH M,DICAIRE A,WAKIL AE,et al. Quantitation of hepatitis B virus(HBV)covalently closed circular DNA(cccDNA)in the liver of HBV-infected patients by LightCycler real-time PCR[J]. J Virol Methods,2004,118(2):159-167.
    [8] CAI D,NIE H,YAN R,et al. A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures[J]. Methods Mol Biol,2013,1030:151-161.
    [9] VOLZ T,ALLWEISS L,BEN MM,et al. The entry inhibitor Myrcludex-B efficiently blocks intrahepatic virus spreading in humanized mice previously infected with hepatitis B virus[J]. J Hepatol,2013,58(5):861-867.
    [10] KOCK J,SCHLICHT HJ. Analysis of the earliest steps of hepadnavirus replication:Genome repair after infectious entry into hepatocytes does not depend on viral polymerase activity[J].J Virol,1993,67(8):4867-4874.
    [11] ADDISON WR,WONG WW,FISCHER KP,et al. A quantitative competitive PCR assay for the covalently closed circular form of the duck hepatitis B virus[J]. Antiviral Res,2000,48(1):27-37.
    [12] XU CH,LI ZS,DAI JY,et al. Nested real-time quantitative polymerase chain reaction assay for detection of hepatitis B virus covalently closed circular DNA[J]. Chin Med J(Engl),2011,124(10):1513-1516.
    [13] WONG DK,HUANG FY,LAI CL,et al. Occult hepatitis B infection and HBV replicative activity in patients with cryptogenic cause of hepatocellular carcinoma[J]. Hepatology,2011,54(3):829-836.
    [14] GUO Y,SHENG S,NIE B,et al. Development of magnetic capture hybridization and quantitative polymerase chain reaction for hepatitis B virus covalently closed circular DNA[J].Hepat Mon,2015,15(1):e23729.
    [15] MU D,YAN L,TANG H,et al. A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system[J]. Biotechnol Lett,2015,37(10):2063-2073.
    [16] HUANG JT,YANG Y,HU YM,et al. A highly sensitive and robust method for hepatitis B virus covalently closed circular DNA detection in single cells and serum[J]. J Mol Diagn,2018,20(3):334-343.
    [17] BAUMERT TF,MEREDITH L,NI Y,et al. Entry of hepatitis B and C viruses-recent progress and future impact[J]. Curr Opin Virol,2014,4:58-65.
    [18] LAMAS LONGARELA,SCHMIDT TT,SCHONEWEIS K,et al.Proteoglycans act as cellular hepatitis delta virus attachment receptors[J]. PLo S One,2013,8(3):e58340.
    [19] ZHONG Y,HAN J,ZOU Z,et al. Quantitation of HBV covalently closed circular DNA in micro formalin fixed paraffin-embedded liver tissue using rolling circle amplification in combination with real-time PCR[J]. Clin Chim Acta,2011,412(21-22):1905-1911.
    [20] ZHONG Y,HU S,XU C,et al. A novel method for detection of HBVcccDNA in hepatocytes using rolling circle amplification combined with in situ PCR[J]. BMC Infect Dis,2014,14:608.
    [21] YEH CT,CHIU HT,CHU CM,et al. G1 phase dependent nuclear localization of relaxed-circular hepatitis B virus DNA and aphidicolin-induced accumulation of covalently closed circular DNA[J]. J Med Virol,1998,55(1):42-50.
    [22] ZHANG X,LU W,ZHENG Y,et al. In situ analysis of intrahepatic virological events in chronic hepatitis B virus infection[J]. J Clin Invest,2016,126(3):1079-1092.
    [23] LAI CL,WONG D,IP P,et al. Reduction of covalently closed circular DNA with long-term nucleos(t)ide analogue treatment in chronic hepatitis B[J]. J Hepatol,2017,66(2):275-281.
    [24] LIU S,ZHOU B,VALDES JD,et al. Serum hepatitis B virus RNA:A new potential biomarker for chronic hepatitis B virus infection[J]. Hepatology,2019,69(4):1816-1827.
    [25] MAK LY,WONG DK,CHEUNG KS,et al. Review article:Hepatitis B core-related antigen(HBcrAg):An emerging marker for chronic hepatitis B virus infection[J]. Aliment Pharmacol Ther,2018,47(1):43-54.
    [26] WOODDELL CI,YUEN MF,CHAN HL,et al. RNAi-based treatment of chronically infected patients and chimpanzees reveals that integrated hepatitis B virus DNA is a source of HBs Ag[J]. Sci Transl Med,2017,9(409). pii:eaan0241.
    [27] CAVIGLIA GP,ABATE ML,TANDOI F,et al. Quantitation of HBV cccDNA in anti-HBc-positive liver donors by droplet digital PCR:A new tool to detect occult infection[J]. J Hepatol,2018,69(2):301-307.
    [28] ALLWEISS L,DANDRI M. The role of cccDNA in HBV maintenance[J]. Viruses,2017,9(6). pii:e156.
    [29] NASSAL M. HBV cccDNA:Viral persistence reservoir and key obstacle for a cure of chronic hepatitis B[J]. Gut,2015,64(12):1972-1984.
    [30] CHEN L,WU C,FAN X,et al. Replication and infectivity of hepatitis B virus in HBV-related glomerulonephritis[J]. Int J Infect Dis,2009,13(3):394-398.
    [31] YU MM,GU XJ,XIA Y,et al. Relationship between HBV cccDNA expression in the human ovary and vertical transmission of HBV[J]. Epidemiol Infect,2012,140(8):1454-1460.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1521) PDF downloads(498) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return