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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 35 Issue 9
Sep.  2019
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Article Navigation >  Journal of Clinical Hepatology  > 2019  >  35(9): 1946-1949

Measurement and clinical significance of activating transcription factor 6 level in peripheral blood in patients with chronic HBV infection

DOI: 10.3969/j.issn.1001-5256.2019.09.012

  • Department of Infectious Diseases, The First People's Hospital of Kunshan

Research funding:

 

  • Received Date: 2019-04-17
  • Published Date: 2019-09-20
    Abstract
  • Objective To investigate the level of activating transcription factor 6 ( ATF6) in peripheral blood and its association with clinical indices in patients with chronic HBV infections. Methods A total of 20 chronic asymptomatic HBV carriers ( ASCs) , 20 patients with chronic hepatitis B ( CHB) , and 20 patients with hepatitis B liver cirrhosis ( LC) , who were treated in The First People's Hospital of Kunshan from March 2016 to February 2019, were enrolled, and 20 healthy individuals who underwent physical examination during the same period of time were enrolled as normal control group ( NC group) . Quantitative real-time PCR was used to measure the mRNA expression of ATF6 in peripheral blood leukocytes for all patients, and its correlation with clinical indices was analyzed. ELISA was used to measure the serum level of ATF6 protein for healthy controls and CHB patients. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups; the independent samples t-test was used for comparison between two groups. Results The ASC, CHB, and LC groups had significantly higher mRNA expression of ATF6 in peripheral blood leukocytes than the NC group ( 1. 99 ± 0. 70/2. 12 ± 0. 75/1. 97 ± 0. 85 vs 0. 71 ± 0. 26, all P <0. 05) . There were no significant differences in the mRNA expression of ATF6 between the individuals with different levels of HBsAg, HBV DNA, aminotransferases, and total bilirubin and with the presence or absence of HBeAg in each group ( all P > 0. 05) . The CHB group had a significantly higher serum level of ATF6 protein than the NC group ( 7. 66 ± 1. 94 ng/ml vs 3. 29 ± 0. 30 ng/ml, t =-9. 971, P <0. 001) . Conclusion ATF6 has a higher expression level in peripheral blood in patients with chronic HBV infection. It may be involved in the course of chronic HBV infection.

     

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