中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 6
Jun.  2017
Turn off MathJax
Article Contents

Inhibitory effect of microRNA-149-5p in the proliferation and migration of HepG2 and Bel-7402 hepatoma cells

DOI: 10.3969/j.issn.1001-5256.2017.06.022
  • Published Date: 2017-06-20
  • Objective To investigate the expression of microRNA-149-5p (miR-149-5p) in liver cancer tissue and its molecular and biological role in hepatoma cells. Methods A total of 65 liver cancer tissue samples and corresponding adjacent tissue samples were collected from January 2010 to January 2014, in the Affiliated Hospital of Inner Mongolia Medical University. Quantitative real-time PCR was used to measure the expression of miR-149-5p in liver cancer tissue and corresponding adjacent tissue. The cells were divided into two groups; the cells in the experimental group were transfected with miR-149-5p mimic, and those in the control group were transfected with the negative control of the mimic. MTT colorimetry and wound-healing assay were performed to determine the effect of miR-149-5p on the proliferation and migration of HepG2 and Bel-7402 cells. The t-test or a one-way analysis of variance was used for comparison of continuous data between groups. Results The liver cancer tissue had significantly lower expression of miR-149-5p than the adjacent tissue (0. 14 ± 0. 06 vs 2. 56 ± 0. 42, t = 7. 79, P < 0. 05) . There were significantly differences in the expression of miR-149-5p in Hep G2 (1. 43 ± 0. 25) 、Bel-7402 (1. 77 ± 0. 32) , and the normal hepatic epithelial cells (5. 68 ± 0. 74) (F = 11. 27, P < 0. 05) . The in vitro functional experiment showed that miR-149-5p mimic significantly inhibited the proliferation of 24、48、72 hour of Hep G2 and Bel-7402 hepatoma cells (Hep G2: t = 4. 98, 5. 17, 7. 78, all P < 0. 05; Bel-7402: t = 6. 83, 7. 09, 15. 67, all P < 0. 05) and inhibited the migration of Hep G2 and Bel-7402 hepatoma cells (t = 23. 11, 17. 42, both P < 0. 05) . Conclusion The expression of miR-149-5p is downregulated in liver cancer tissue, and overexpressed miR-149-5p can inhibit the proliferation and migration of hepatoma cells, suggesting that miR-149-5p may be a promising and effective molecular target for the genetic treatment of liver cancer.

     

  • loading
  • [1]SIEGEL RL, MILLER KD, JEMAL A.Cancer Statistics, 2016[J].CA Cancer J Clin, 2016, 66 (1) :7-30.
    [2]HUANG J.Current progress in epigenetic research for hepatocarcinomagenesis[J].Sci China C Life Sci, 2009, 52 (1) :31-42.
    [3]ZHAO LJ, LI ZF, SHI RY, et al.Expression of NOB1 in liver cancer tissues and its significance[J].J Clin Hepatol, 2016, 32 (4) :739-741. (in Chinese) .赵丽娟, 李志锋, 石荣亚, 等.NOB1在肝癌组织中的表达及意义[J].临床肝胆病杂志, 2016, 32 (4) :739-741.
    [4]BARTEL DP.Micrornas:genomics, biogenesis, mechanism, and function[J].Cell, 2004, 116 (2) :281-297.
    [5]PASQUINELLI AE.Micrornas and their targets:recognition, regulation and an emerging reciprocal relationship[J].Nat Rev Genet, 2012, 13 (4) :271-282.
    [6]HWANG HW, MENDELL JT.Micrornas in cell proliferation, cell death, and tumorigenesis[J].Br J Cancer, 2006, 94 (6) :776-780.
    [7]SCHICKEL R, BOYERINAS B, PARK SM, et al.Micrornas:key players in the immune system, differentiation, tumorigenesis and cell death[J].Oncogene, 2008, 27 (45) :5959-5974.
    [8]MENDELL JT.Micrornas:critical regulators of development, cellular physiology and malignancy[J].Cell Cycle, 2005, 4 (9) :1179-1184.
    [9]LI ZW, WU T, FU YX, et al.Expression and roles of MicroRNA-31 in HCC[J].Chin J Clin Pharmacol Ther, 2015, 20 (10) :1098-1101. (in Chinese) 李中文, 吴涛, 付应霄, 等.MicroRNA-31在肝癌中的表达和作用[J].中国临床药理学与治疗学, 2015, 20 (10) :1098-1101.
    [10]JIN L, LI Y, LIU J, et al.Tumor suppressor mir-149-5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma[J].Mol Med Rep, 2016, 13 (6) :5386-5392.
    [11]XU K, LIU X, MAO X, et al.Microrna-149 suppresses colorectal cancer cell migration and invasion by directly targeting forkhead box transcription factor foxm1[J].Cell Physiol Biochem, 2015, 35 (2) :499-515.
    [12]CHAN SH, HUANG WC, CHANG JW, et al.Microrna-149 targets git1 to suppress integrin signaling and breast cancer metastasis[J].Oncogene, 2014, 33 (36) :4496-4507.
    [13] SCHMITTGEN TD, LIVAK KJ.Analyzing Real-Time PCR data by the comparative C (T) method[J].Nat Protoc, 2008, 3 (6) :1101-1108.
    [14]WONG CM, NG IO.Molecular pathogenesis of hepatocellular carcinoma[J].Liver Int, 2008, 28 (2) :160-174.
    [15]MORENO-MOYA JM, VILELLA F, SIMON C.Microrna:key gene expression regulators[J].Fertil Steril, 2014, 101 (6) :1516-1523.
    [16]CALIN GA, CROCE CM.Microrna signatures in human cancers[J].Nat Rev Cancer, 2006, 6 (11) :857-866.
    [17]MEDINA PP, SLACK FJ.Micrornas and cancer:an overview[J].Cell Cycle, 2008, 7 (16) :2485-2492.
    [18]NIKITINA EG, URAZOVA LN, STEGNY VN.Micrornas and human cancer[J].Exp Oncol, 2012, 34 (1) :2-8.
    [19]FARAZI TA, HOELL JI, MOROZOV P, et al.Micrornas in human cancer[J].Adv Exp Med Biol, 2013, 774:1-20.
    [20]SHENOUDA SK, ALAHARI SK.Microrna function in cancer:oncogene or a tumor suppressor?[J].Cancer Metastasis Rev, 2009, 28 (3-4) :369-378.
    [21]FENG M, E CY, LI H, et al.Expression of miR-196a in liver cancer cells and its promotion effect on proliferation[J].J Jilin Univ:Med Edit, 2015, 41 (1) :120-124. (in Chinese) 冯默, 鄂长勇, 李航, 等.MiR-196a在肝细胞癌中的表达及其促增殖作用[J].吉林大学学报:医学版, 2015, 41 (1) :120-124.
    [22]NANA-SINKAM SP, CROCE CM.Micrornas as therapeutic targets in cancer[J].Transl Res, 2011, 157 (4) :216-225.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2401) PDF downloads(429) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return