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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 32 Issue 1
Jan.  2016
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Article Contents

Application of CRISPR/Cas9 system in virus research

DOI: 10.3969/j.issn.1001-5256.2016.01.041
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  • Received Date: 2015-05-07
  • Published Date: 2016-01-20
  • To date,the clustered regularly interspaced short palindromic repeat( CRISPR) / Cas9 system has been widely used to edit the genome in many species and cells. The system is the third generation of artificial endonuclease,which can edit DNA by recognizing short DNA sequences. This paper reviews the structural features of the system and its application in virus research,such as the functional studies of virus- related genes and the exploration of antiviral therapies( including HIV,HBV,and EB virus),looking forward to the future direction of virus research.

     

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  • [1] BIBIKOVA M,BEUMER K,TRAUTMAN JK,et al.Enhancing gene targeting with designed zinc finger nucleases[J].Science,2003,300(5620):764-764.
    [2] BIBIKOVA M,GOLIC M,GOLIC KG,et al.Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases[J].Genetics,2002,161(3):1169-1175.
    [3]IRION U,KRAUSS J,NSSLEIN-VOLHARD C.Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system[J].Development,2014,141(24):4827-4830.
    [4]RYAN OW,CATE JH.Multiplex engineering of industrial yeast genomes using CRISPRm[J].Methods Enzymol,2014,546:473-489.
    [5]QIN W,DION SL,KUTNY PM,et al.Efficient CRISPR/Cas9-mediated genome editing in mice by zygote electroporation of nuclease[J].Genetics,2015,200(2):423-430.
    [6]JIANG W,ZHOU H,BI H,et al.Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis,tobacco,sorghum and rice[J].Nucleic Acids Res,2013,41(20):e188.
    [7]GRISSA I,VERGNAUD G,POURCEL C.The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats[J].BMC Bioinformatics,2007,8:172.
    [8]GARNEAU JE,DUOUIS ME,VILLION M,et al.The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA[J].Nature,2010,468(7320):67-71.
    [9]DELTCHEVA E,CHYLINSKI K,SHARMA CM,et al.CRISPR RNA maturation by trans-encoded small RNA and host factor RNaseⅢ[J].Nature,2011,471(7340):602-607.
    [10] CONG L,RAN FA,COX D,et al.Multiplex genome engineering using CRISPR/Cas systems[J].Science,2013,339(6121):819-823.
    [11]SUENAGE T,KOHYAMA M,HIRAYASU K,et al.Engineering large viral DNA genomes using the CRISPR-Cas9 system[J].Microbiol Immunol,2014,58(9):513-522.
    [12]JOHNSON KE,BOTTERO V,FLAHERTY S,et al.IFI16 restricts HSV-1 replication by accumulating on the hsv-1 genome,repressing HSV-1 gene expression,and directly or indirectly modulating histone modifications[J].PLo S Pathog,2014,10(11):e1004503.
    [13]BI Y,SUN L,GAO D,et al.High-efficiency targeted editing of large viral genomes by RNA-guided nucleases[J].PLo S Pathog,2014,10(5):e1004090.
    [14]ROBLES-SIKISAKA R,LY M,BOEHM T,et al.Association between living environment and human oral viral ecology[J].ISME J,2013,7(9):1710-1724.
    [15]GU WG,CHEN XQ.Targeting CCR5 for anti-HIV research[J].Eur J Clin Microbiol Infect Dis,2014,33(11):1881-1887.
    [16]HU W,KAMINSKI R,YANG F,et al.RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection[J].Proce Nati Acad Sci U S A,2014,111(31):11461-11466.
    [17]SCHIFFER JT,SWAN DA,STONE D,et al.Predictors of hepatitis B cure using gene therapy to deliver DNA cleavage enzymes:a mathematical modeling approach[J].PLo S Comput Biol,2013,9(7):e1003131.
    [18]LIN SR,YANG HC,KUO YT,et al.The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo[J].Mol Ther Nucleic Acids,2014,3:e186.
    [19]DONG C,QU L,WANG H,et al.Targeting hepatitis B virus ccc DNA by CRISPR/Cas9 nuclease efficiently inhibits viral replication[J].Antiviral Res,2015,118:110-117.
    [20]SEEGER C,SOHN JA.Targeting hepatitis B virus with CRISPR/Cas9[J].Mol Ther Nucleic Acids,2014,3(12):e216.
    [21]ZHEN S,HUA L,LIU YH,et al.Harnessing the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated Cas9 system to disrupt the hepatitis B virus[J].Gene Ther,2015,22(5):402-412.
    [22]KENNEDY EM,BASSIT LC,MUELLER H,et al.Suppression of hepatitis B virus DNA accumulation in chronically infected cells using a bacterial CRISPR/Cas RNA-guided DNA endonuclease[J].Virology,2015,476:196-205.
    [23]VOCKERODT M,YAP LF,SHANNON-LOWE C,et al.The Epstein-Barr virus and the pathogenesis of lymphoma[J].J Pathol,2015,235(2):312-322.
    [24]WANG J,QUAKE SR.RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection[J].Proc Natl Acad Sci U S A,2014,111(36):13157-13162.
    [25]CHO SW,KIM S,KIM Y,et al.Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases[J].Genome Res,2014,24(1):132-141.
    [26]GHEZRAOUI H,PIGANEAU M,RENOUF B,et al.Chromosomal translocations in human cells are generated by canonical nonhomologous end-joining[J].Mol Cell,2014,55(6):829-842.
    [27]TSAI SQ,WYVEKENS N,KHAYTER C,et al.Dimeric CRISPR RNA-guided Fokl nucleases for highly specific genome editing[J].Nat Biotechnol,2014,32(6):569-576.
    [28]RAMAKRISHNA S,KWAKU DAD AB,BELOOR J,et al.Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA[J].Genome Res,2014,24(6):1020-1027.
    [29]KOTTERMAN MA,SCHAFFER DV.Engineering adeno-associated viruses for clinical gene therapy[J].Nat Rev Genet,2014,15(7):445-451.
    [30]KIM S,KIM D,CHO SW,et al.Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins[J].Genome Res,2014,24(6):1012-1019.
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