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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 40 Issue 2
Feb.  2024
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Article Navigation >  Journal of Clinical Hepatology  > 2024  >  40(2): 312-318

Protective mechanism of rhubarb decoction against inflammatory damage of brain tissue in rats with mild hepatic encephalopathy： A study based on the PI3K/AKT/mTOR signaling pathway

DOI: 10.12449/JCH240215
  • 1.

    Graduate School of Guangxi University of Chinese Medicine，Nanning 530200，China

  • 2.

    Department of Scientific Research，Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine，Nanning 530011，China

  • 3a.

    Department of Hepatology，The First Affiliated Hospital of Guangxi University of Chinese Medicine，Nanning 530023，China

  • 3b.

    Department of Spleen and Stomach，The First Affiliated Hospital of Guangxi University of Chinese Medicine，Nanning 530023，China

  • 3c.

    Laboratory of Molecular Biology，The First Affiliated Hospital of Guangxi University of Chinese Medicine，Nanning 530023，China

  • 3d.

    Department of Oncology，The First Affiliated Hospital of Guangxi University of Chinese Medicine，Nanning 530023，China

Research funding:

National Natural Science Foundation of China (82260899);

National Natural Science Foundation of China (82060848);

National Natural Science Foundation of China (81804019);

Guangxi Natural Science Foundation Key Project (Gui Ke AB22035076);

Guangxi Natural Science Foundation Project (2020GXNSFAA297070);

Cultivation of Guangxi First-class Disciplines in Integrative Medicine (Gui Ke Research［2018］No.12);

Cultivation of Guangxi First-class Disciplines in Integrative Medicine (2019XK145);

Guangxi Postgraduate Education Innovation Programme Funded Projects (YCSW2022349);

Guangxi Postgraduate Education Innovation Programme Funded Projects (YCSY2023033);

Guangxi Postgraduate Education Innovation Programme Funded Projects (YCSZ2022017)

More Information
  • Corresponding author: MAO Dewen， mdwboshi2005@163.com （ORCID： 0000-0001-9438-9325）; YAO Chun， yaochunlshn@163.com （ORCID： 0000-0003-2903-8814）
  • Received Date: 2023-05-19
  • Accepted Date: 2023-07-04
  • Published Date: 2024-02-19
    Abstract
  •   Objective  To investigate the role and possible mechanism of action of rhubarb decoction （RD） retention enema in improving inflammatory damage of brain tissue in a rat model of mild hepatic encephalopathy （MHE）.  Methods  A total of 60 male Sprague-Dawley rats were divided into blank group （CON group with 6 rats） and chronic liver cirrhosis modeling group with 54 rats using the complete randomization method. After 12 weeks， 40 rats with successful modeling which were confirmed to meet the requirements for MHE model by the Morris water maze test were randomly divided into model group （MOD group）， lactulose group （LT group）， low-dose RD group （RD1 group）， middle-dose RD group （RD2 group）， and high-dose RD group （RD3 group）， with 8 rats in each group. The rats in the CON group and the MOD group were given retention enema with 2 mL of normal saline once a day； the rats in the LT group were given retention enema with 2 mL of lactulose at a dose of 22.5% once a day； the rats in the RD1， RD2， and RD3 groups were given retention enema with 2 mL RD at a dose of 2.5， 5.0， and 7.5 g/kg， respectively， once a day. After 10 days of treatment， the Morris water maze test was performed to analyze the spatial learning and memory abilities of rats. The rats were analyzed from the following aspects： behavioral status； the serum levels of alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， interleukin-1β （IL-1β）， interleukin-6 （IL-6）， and tumor necrosis factor-α （TNF-α） and the level of blood ammonia； pathological changes of liver tissue and brain tissue； the mRNA and protein expression levels of phosphatidylinositol 3-kinase （PI3K）， protein kinase B （AKT）， and mammalian target of rapamycin （mTOR） in brain tissue. A one-way analysis of variance was used for comparison of continuous data between multiple groups， and the least significant difference t-test was used for further comparison between two groups.  Results  Compared with the MOD group， the RD1， RD2， and RD3 groups had a significantly shorter escape latency （all P<0.01）， significant reductions in the levels of ALT， AST， IL-1β， IL-6， TNF-α， and blood ammonia （all P<0.05）， significant alleviation of the degeneration， necrosis， and inflammation of hepatocytes and brain cells， and significant reductions in the mRNA and protein expression levels of PI3K， AKT， and mTOR in brain tissue （all P<0.05）， and the RD3 group had a better treatment outcome than the RD1 and RD2 groups.  Conclusion  Retention enema with RD can improve cognitive function and inflammatory damage of brain tissue in MHE rats， possibly by regulating the PI3K/AKT/mTOR signaling pathway.

     

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