非酒精性脂肪性肝炎大鼠模型的建立及其胰腺、肾脏的组织学病理变化

宋清莲; 谭玉娥; 刘京京; 袁贝贝; 李敏; 贺礼琴; 戴光荣

doi:10.3969/j.issn.1001-5256.2020.01.031

非酒精性脂肪性肝炎大鼠模型的建立及其胰腺、肾脏的组织学病理变化

DOI: 10.3969/j.issn.1001-5256.2020.01.031

延安大学附属医院消化内科

基金项目:

陕西省社会发展科技攻关项目(2016SF-120)；

详细信息

中图分类号: R575;R-332
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出版历程
- 收稿日期: 2019-09-09
- 出版日期: 2020-01-20

Establishment of a rat model of nonalcoholic steatohepatitis and histopathological changes of the pancreas and the kidney

Department of Gastroenterology,Yan'an University Affiliated Hospital

Research funding:

摘要

摘要: 目的探讨非酒精性脂肪性肝炎大鼠模型肝外脏器胰腺、肾脏的组织学病理变化情况。方法将20只SD大鼠中的6只首先按完全随机配对分配到观察组（B组）,将剩下的14只按体质量配对成7个区组,最后将每个区组中的1只大鼠按随机数字表分配到实验组（C组）,其余7只为对照组（A组）。B组和C组给予高脂饮食,A组给予普通饮食。B组于实验第4、8、12周各处死2只,提取肝脏组织行HE染色,显微镜下观察肝脏病理切片是否达到脂肪性肝炎,第12周末建模成功（提示C组建模成功）。处死A组和C组全部大鼠,分别检测血清学指标ALT、AST、GGT、TG、TC、LDL、GLU、INS、HOMA-IR,取肝脏组织行HE染色;胰腺组织行HE染色观察大鼠胰腺腺泡和胰岛变化情况;肾脏组织行PAS染色,免疫组织化学desmin蛋白染色观察肾小球和足细胞desmin表达情况。计量资料2组间比较采用t检验。结果 C组大鼠病理评分及血清ALT、GGT、LDL、TG、TC、GLU、INS、HOMAIR水平均高于A组（t值分别为4. 67、2. 83、2. 34、4. 58、4. 78、3. 00、2. 97、3. 80、4. 10,P值均...
- 非酒精性脂肪性肝炎 /
- 大鼠,Sprague-Dawley /
- 胰腺 /
- 肾脏 /
- 组织和显徽镜检结果
Abstract: Objective To establish a rat model of nonalcoholic steatohepatitis,and to investigate the histopathological changes of the extrahepatic organs of the pancreas and the kidney. Methods A total of 20 Sprague-Dawley rats were selected,among which 6 were randomly selected as the observation group(group B). The remaining 14 rats were paired into seven groups according to body weight,then a random number table was used to select 1 rat from each group and assign them into the experimental group(group C),and the remaining 7 rats were assigned to the control group(group A). The rats in groups B and C were given high-fat diet,while those in group A were given a normal diet. In group B,two rats each were killed at weeks 4,8,and 12 of the experiment,liver tissue was extracted for HE staining,and liver pathological sections were observed under a microscope to see whether steatohepatitis was achieved. The model was successfully established at the end of week 12. All rats in groups A and C were sacrificed,and the serological markers alanine aminotransferase(ALT),aspartate aminotransferase(AST),gamma-glutamyl transpeptidase(GGT),triglyceride(TG),total cholesterol(TC),low-density lipoprotein(LDL),glucose(GLU),insulin(INS),and homeostasis model assessment of insulin resistance(HOMA-IR) were measured. HE staining was performed for liver tissue and pancreatic tissue to observe the changes in pancreatic acini and pancreatic islets. PAS staining was performed for kidney tissue,and immunohistochemical desmin-staining was performed to observe the expression of desmin in glomerular podocytes. The t-test was used for comparison of continuous data between two groups. Results Compared with group A,group C had significantly higher pathological score and serum levels of ALT,GGT,LDL,TG,TC,GLU,INS,and HOMA-IR(t = 4. 67,2. 83,2. 34,4. 58,4. 78,3. 00,2. 97,3. 80 and 4. 10,all P < 0. 05). Liver pathological changes were observed in the high-fat group from week 4 to week 12,i. e.,the lobular structure was gradually destroyed,the small fat vacuoles in the cytoplasm of the hepatocytes gradually developed into large fat vacuoles,and inflammatory cell infiltration gradually appeared in the portal area. Pancreatic pathological changes were observed in the high-fat group at the end of week 12,i. e.,apoptosis and atrophy of pancreatic acinar cells were observed,and no significant abnormalities were observed in pancreatic islet cells. Renal pathological changes were observed in the high-fat group at the end of week 12,i. e.,therewere significant increases in the substances with positive PAS staining results,the glomerular volume,and the thickness of the glomerular capillary basement membrane,with disordered arrangement of capillary loops,hyperplasia of the mesangial area,swelling of renal tubular epithelial cells,and stenosis of some lumens. Immunohistochemical staining showed an increase in the expression of desmin in glomerular podocytes in the high-fat group. Conclusion A rat model of nonalcoholic steatohepatitis is successfully established by high-fat diet.Nonalcoholic steatohepatitis in rats may lead to apoptosis and atrophy of pancreatic acinar cells,increase in glomerular volume,thickening of the capillary basement membrane,mesangial hyperplasia,and podocyte injury.
- non-alcoholic steatohepatitis /
- rats,sprague-dawley /
- pancreas /
- kidney /
- morphological and microscopic findings

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参考文献(24)

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