CD38和MUM-1在自身免疫性肝炎中的表达及临床意义

蒋丽琳; 汤林鑫; 顾娟; 陆忠华; 汤鸿; 王若飞

doi:10.3969/j.issn.1001-5256.2019.09.026

CD38和MUM-1在自身免疫性肝炎中的表达及临床意义

DOI: 10.3969/j.issn.1001-5256.2019.09.026

1. 无锡市第五人民医院病理科
2. 无锡市第五人民医院肝病科
3. 无锡市第二人民医院病理科
4. 武警江苏总队医院传染科

基金项目:

无锡市科教强卫工程项目(ZDRC026)；

详细信息

中图分类号: R575.1
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出版历程
- 收稿日期: 2019-05-30
- 出版日期: 2019-09-20

Expression and clinical significance of CD38 and multiple myeloma oncogene 1 in autoimmune hepatitis

Department of Pathology, Wuxi Fifth People's Hospital

Research funding:

摘要

摘要:
目的观察自身免疫性肝炎（AIH）患者肝穿刺组织中白细胞分化抗原38（CD38）和多发性骨髓瘤癌基因1（MUM-1）的表达,探讨其在AIH发生发展中的作用。方法以2017年1月-2018年12月无锡市第五人民医院病理科46例AIH患者肝穿组织作为实验组,以30例胆囊癌根治标本的正常肝组织为对照组,采用免疫组化法检测CD38和MUM-1的表达,并结合患者的临床和病理特征进行分析。非正态性计量资料2组间比较采用Wilcoxon秩和检验;计数资料2组间比较采用χ2检验;相关性分析采用Spearman秩相关分析法。结果 46例AIH患者的CD38和MUM-1的阳性表达均高于对照组（Z值分别为-7. 181、-7. 484,P值均<0. 001）。在AIH患者中炎症活动分级≥3级和纤维化4期CD38和MUM-1的阳性表达均显著增加,与炎症活动分级<3级和纤维化<4期相比,差异均有统计学意义（χ2值分别为10. 085、15. 769、14. 988、26. 966,P值均<0. 05）。CD38和MUM-1的表达与慢性炎症活动分级及纤维化分期呈显著正相关（r值分别为0. 5...
- 肝炎,自身免疫性 /
- 抗原,CD38 /
- 干扰素调节因子类 /
- 病理学,分子
Abstract:
Objective To investigate the expression of the leukocyte differentiation antigen CD38 and multiple myeloma oncogene 1 ( MUM-1) in liver biopsy tissue of patients with autoimmune hepatitis ( AIH) , as well as their roles in the development and progression of AIH. Methods Liver biopsy tissue samples from 46 AIH patients who were admitted to Department of Pathology in Wuxi Fifth People's Hospital from January 2017 to December 2018 were selected as experimental group, and normal liver tissue samples from 30 patients with gallbladder carcinoma who underwent radical treatment were selected as control group. Immunohistochemistry was used to measure the expression of CD38 and MUM-1, and an analysis was performed with reference to patients' clinical and pathological features. The Wilcoxon rank-sum test was used for comparison of non-normally distributed continuous data between two groups, the chi-square test was used for comparison of categorical data between groups, and a Spearman correlation analysis was also performed. Results The experimental group had significantly higher expression of CD38 and MUM-1 than the control group ( Z =-7. 181 and-7. 484, both P < 0. 001) . The AIH patients with inflammatory activity grade ≥3 and fibrosis stage 4 had significant increases in the expression of CD38 and MUM-1, with significantly higher expression than those with inflammatory activity grade < 3 and fibrosis stage < 4 ( χ2= 10. 085, 15. 769, 14. 988, and26. 966, all P < 0. 05) . The expression of CD38 and MUM-1 was positively correlated with chronic inflammatory activity grade and fibrosis stage ( r = 0. 552, 0. 876, 0. 603, and 0. 934, all P < 0. 001) , and CD38 was also positively correlated with MUM-1 ( r = 0. 932, P <0. 001) . Conclusion CD38 and MUM-1 are involved in the inflammatory activity of AIH and the process of fibrosis formation. There is a synergistic effect between them, which affects the process of liver cirrhosis. Therefore, they may become potential targets for diagnosis and treatment in future.
- hepatitis, autoimmune /
- antigens, CD38 /
- interferon regulatory factors /
- pathology, molecular

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